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Here, an isothermal microcalorimeter, equipped with a vessel holder similar to a 48\u2010well plate, was used. The increased throughput of this microcalorimeter makes it valuable for biomedical and pharmaceutical applications. Our results show that the sensitivity of the instrument allows the detection of 3 \u00d7 10<jats:sup>4<\/jats:sup> bacteria per vial. Growth of <jats:italic>P. mirabilis<\/jats:italic> in Luria Broth medium was detected between 2 and 9 h with decreasing inoculum. The culture released 2.1J with a maximum thermal power of 76 \u03bcW. The growth rate calculated using calorimetric and spectrophotometric data were 0.60 and 0.57 h<jats:sup>\u20131<\/jats:sup>, respectively. Additional insight on protease activities of <jats:italic>P. mirabilis<\/jats:italic> matching the last peak in heat production could be gathered as well. Growth of tumor microtissues releasing a maximum thermal power of 2.1 \u03bcW was also monitored and corresponds to a diameter increase of the microtissues from ca. 100 to 428 \u03bcm. This opens new research avenues in cancer research, diagnostics, and development of new antitumor drugs. For parasitic worms, the technique allows assessment of parasite survival using motor and metabolic activities even with a single worm.<\/jats:p>","DOI":"10.1002\/biot.201400494","type":"journal-article","created":{"date-parts":[[2014,12,15]],"date-time":"2014-12-15T11:26:44Z","timestamp":1418642804000},"page":"460-468","source":"Crossref","is-referenced-by-count":75,"title":["Isothermal microcalorimetry accurately detects bacteria, tumorous microtissues, and parasitic worms in a label\u2010free well\u2010plate assay"],"prefix":"10.1002","volume":"10","author":[{"given":"Olivier","family":"Braissant","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer","family":"Keiser","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabel","family":"Meister","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Bachmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dieter","family":"Wirz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beat","family":"G\u00f6pfert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gernot","family":"Bonkat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ingemar","family":"Wads\u00f6","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,2,18]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0040-6031(02)00263-0"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-410X.2011.10902.x"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/jor.22419"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-7-116"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02374-08"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02374-13"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1385\/BTER:75:1-3:167"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-022X(98)00050-5"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0040-6031(01)00825-5"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10973-007-8680-9"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tifs.2005.01.008"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fm.2011.08.018"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tca.2013.02.013"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodcont.2008.11.008"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10856-011-4400-x"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2012.11.020"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2010.08.011"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182011001934"},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2009.01819.x"},{"key":"e_1_2_7_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0142-9612(02)00317-4"},{"key":"e_1_2_7_22_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00168.2012"},{"key":"e_1_2_7_23_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00431084"},{"key":"e_1_2_7_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/0040-6031(94)02062-S"},{"key":"e_1_2_7_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/0040-6031(93)80322-2"},{"key":"e_1_2_7_26_2","unstructured":"Kemp R. 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