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A large part of the attraction of CRISPR stems from the small size of its two core components \u2013 Cas9 and gRNA \u2013 and hence its compatibility with virtually any available viral vector delivery system. As a result, over the past two years, four major classes of viral vectors have already been engineered and applied as CRISPR delivery tools \u2013 retroviruses, lentiviruses, adenoviruses, and adeno\u2010associated viruses (AAVs). The juxtaposition of these two technologies reflects a case of tremendous mutual attraction and holds unprecedented promises for biology and medicine. Here, we provide an overview of the state\u2010of\u2010the\u2010art of this rapidly emerging field, from a comparative description of the principal vector designs, to a synopsis of some of the most exciting applications that were reported to date, including the use of viral CRISPR vectors for genome\u2010wide loss\u2010of\u2010function screens, multiplexed gene editing or disease modeling in animals. Once specificity and safety have been improved further, viral vector\u2010mediated in vitro\/in vivo CRISPR delivery and expression promise to radically transform basic and applied biomedical research.<\/jats:p>","DOI":"10.1002\/biot.201400529","type":"journal-article","created":{"date-parts":[[2015,2,6]],"date-time":"2015-02-06T08:57:02Z","timestamp":1423213022000},"page":"258-272","source":"Crossref","is-referenced-by-count":79,"title":["CRISPR genome engineering and viral gene delivery: A case of mutual attraction"],"prefix":"10.1002","volume":"10","author":[{"given":"Florian","family":"Schmidt","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dirk","family":"Grimm","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,2,6]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrg2006"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI34129"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature04791"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1186\/1758-907X-2-8"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.copbio.2012.01.013"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3486"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.coi.2012.08.005"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-biochem-060713-035418"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.341.6148.833"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2014.05.010"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1258096"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.2842"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.1769"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrg1066"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrg2141"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/mt.2009.255"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.2174\/1566523034578285"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/biot.201400046"},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.3055"},{"key":"e_1_2_7_21_2","doi-asserted-by":"publisher","DOI":"10.1089\/hum.2010.245"},{"key":"e_1_2_7_22_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.gt.3301514"},{"key":"e_1_2_7_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2014.09.014"},{"key":"e_1_2_7_24_2","doi-asserted-by":"publisher","DOI":"10.1038\/bjp.2008.349"},{"key":"e_1_2_7_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymthe.2006.05.006"},{"key":"e_1_2_7_26_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1247005"},{"key":"e_1_2_7_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2014.09.008"},{"key":"e_1_2_7_28_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.227132.113"},{"key":"e_1_2_7_29_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gku749"},{"key":"e_1_2_7_30_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.mt.6300382"},{"key":"e_1_2_7_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1046-2023(02)00219-0"},{"key":"e_1_2_7_32_2","doi-asserted-by":"crossref","first-page":"8463","DOI":"10.1128\/JVI.72.11.8463-8471.1998","article-title":"A third\u2010generation lentivirus vector with a conditional packaging system.","volume":"72","author":"Dull T.","year":"1998","journal-title":"J. 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