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In this work we describe the generation of a new human amniocyte\u2010derived cell line named 1G3 and show that it can be used as a very promising cell host for AdV production in serum\u2010free conditions, allowing for production in high cell density cultures and avoiding the typical cell density effect observed for HEK293. By design, this cell line makes the generation of replication\u2010competent adenovirus during production of E1\u2010deleted AdVs very unlikely. The impact of the culture system (static versus agitated) and AdV infection parameters such as multiplicity of infection, time of harvesting and cell concentration at infection were evaluated and compared with HEK293. Using stirred tanks bioreactors, it was possible to grow 1G3 cells to cell densities of up to 9 \u00d7 10<jats:sup>6<\/jats:sup> cells\/mL using serum\u2010free media. Moreover, without a medium exchange step at infection, a three\u2010fold increase in AdV volumetric titers was obtained, as no cell density effect was observed at CCI 3. Overall, our results clearly demonstrate the potential of the human amniocyte\u2010derived newly established cell line 1G3 for AdV production in a serum\u2010free scalable process, paving the way for further process improvements based on fed\u2010batch or perfusion strategies.<\/jats:p>","DOI":"10.1002\/biot.201400765","type":"journal-article","created":{"date-parts":[[2015,5,6]],"date-time":"2015-05-06T05:50:31Z","timestamp":1430891431000},"page":"760-771","source":"Crossref","is-referenced-by-count":9,"title":["Human amniocyte\u2010derived cells are a promising cell host for adenoviral vector production under serum\u2010free conditions"],"prefix":"10.1002","volume":"10","author":[{"given":"Ana Carina","family":"Silva","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Sim\u00e3o","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia","family":"K\u00fcppers","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tanja","family":"Lucas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcos F. 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