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Recently, microfluidic systems have been developed to provide miniaturized platforms for antimicrobial susceptibility assays. However, some of current microfluidic based assays require continuous flows of reagents or elaborate preparation steps during concentration preparation. In this study, we introduce a novel microfluidic chip based growth phenotype assay that automatically generates a logarithmic concentration gradient and allows observing the growth of pathogenic bacteria under different concentrations of antibiotics in nanoliter batch culture reactors. We chose pathogen bacterium <jats:italic>Pseudomonas aeruginosa<\/jats:italic> as a model strain and evaluated the inhibitory effects of gentamicin and ciprofloxacin. We determined the <jats:italic>EC<\/jats:italic><jats:sub>50<\/jats:sub> values and confirmed the validity of the present system by comparing the <jats:italic>EC<\/jats:italic><jats:sub>50<\/jats:sub> values obtained through conventional test tube method. We demonstrated that the <jats:italic>EC<\/jats:italic><jats:sub>50<\/jats:sub> values acquired from present assay are comparable to those obtained from conventional test tube cultures. The potential application of present assay system for investigating combinatorial effects of antibiotics on multidrug resistant pathogenic bacteria is discussed and it can be further used for systematic evaluation of antifungal or antiviral agents.<\/jats:p>","DOI":"10.1002\/biot.201500037","type":"journal-article","created":{"date-parts":[[2015,6,25]],"date-time":"2015-06-25T05:50:15Z","timestamp":1435211415000},"page":"1783-1791","source":"Crossref","is-referenced-by-count":28,"title":["Determination of antibiotic EC<sub>50<\/sub> using a zero\u2010flow microfluidic chip based growth phenotype assay"],"prefix":"10.1002","volume":"10","author":[{"given":"Jing","family":"Dai","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sang\u2010Jin","family":"Suh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Morgan","family":"Hamon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jong Wook","family":"Hong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,7,16]]},"reference":[{"key":"e_1_2_7_2_2","first-page":"697","article-title":"Resistance to antibiotics: Are we in the post\u2010antibiotic era?","volume":"36","author":"Alfonso J A.","year":"2005","journal-title":"Arch. 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