{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T15:30:50Z","timestamp":1782747050341,"version":"3.54.5"},"reference-count":48,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2015,12,29]],"date-time":"2015-12-29T00:00:00Z","timestamp":1451347200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004085","name":"Korean Ministry of Education, Science and Technology","doi-asserted-by":"publisher","award":["2011\u20100031962"],"award-info":[{"award-number":["2011\u20100031962"]}],"id":[{"id":"10.13039\/501100004085","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001321","name":"National Research Foundation","doi-asserted-by":"publisher","award":["2014069603"],"award-info":[{"award-number":["2014069603"]}],"id":[{"id":"10.13039\/501100001321","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotechnology Journal"],"published-print":{"date-parts":[[2016,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>To find a more effective chemical reagent for improved monoclonal antibody (mAb) production, eight chemical reagents (curcumin, quercein, DL\u2010sulforaphane, thymidine, valeric acid, phenyl butyrate, valproic acid, and lithium chloride) known to induce cell cycle arrest were examined individually as chemical additives to recombinant CHO (rCHO) cell cultures producing mAb. Among these chemical additives, valeric acid showed the best production performance. Valeric acid decreased specific growth rate (\u03bc), but increased culture longevity and specific mAb productivity (<jats:italic>q<\/jats:italic><jats:sub>mAb<\/jats:sub>) in a dose\u2010dependent manner. The beneficial effect of valeric acid on culture longevity and <jats:italic>q<\/jats:italic><jats:sub>mAb<\/jats:sub> outweighed its detrimental effect on \u03bc, resulting in 2.9\u2010fold increase in the maximum mAb concentration when 1.5 mM valeric acid was added to the cultures. Furthermore, valeric acid did not negatively affect the mAb quality attributes with regard to aggregation, charge variation, and galactosylation. Unexpectedly, galactosylation of the mAb increased by the 1.5 mM valeric acid addition. Taken together, the results obtained here demonstrate that valeric acid is an effective chemical reagent to increase mAb production in rCHO cells.<\/jats:p>","DOI":"10.1002\/biot.201500327","type":"journal-article","created":{"date-parts":[[2015,12,12]],"date-time":"2015-12-12T21:31:20Z","timestamp":1449955880000},"page":"487-496","source":"Crossref","is-referenced-by-count":71,"title":["Valeric acid induces cell cycle arrest at G1 phase in CHO cell cultures and improves recombinant antibody productivity"],"prefix":"10.1002","volume":"11","author":[{"given":"Jin Hyoung","family":"Park","sequence":"first","affiliation":[{"name":"Department of Biological Sciences, KAIST  Daejeon Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soo Min","family":"Noh","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, KAIST  Daejeon Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ju Rang","family":"Woo","sequence":"additional","affiliation":[{"name":"New Drug Development Center  Cheongju Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jong Won","family":"Kim","sequence":"additional","affiliation":[{"name":"New Drug Development Center  Cheongju Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gyun Min","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, KAIST  Daejeon Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,12,29]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/biot.201400646"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/biot.201400050"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-003-1385-5"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0290(19970920)55:6<927::AID-BIT10>3.0.CO;2-4"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.21758"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1023\/B:BILE.0000009462.10772.a4"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.10566"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10616-007-9059-2"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0093865"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-014-6012-0"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.25332"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-005-1980-8"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.10108"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1021\/bp049807b"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/mutage\/17.5.419"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1012466112116"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1389-1723(01)80114-6"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.21882"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12033-013-9725-x"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0290(2000)71:3<184::AID-BIT1008>3.0.CO;2-W"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-014-5596-8"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1999.0550"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiotec.2004.05.003"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2009.05.042"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2010.02.003"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0290(19980405)58:1<73::AID-BIT8>3.0.CO;2-R"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01024632"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.21589"},{"key":"e_1_2_7_30_1","first-page":"2141","article-title":"Growth\u2010inhibition by thymidine of leukemic Hl\u201060 and normal human myeloid progenitor cells","volume":"41","author":"Akman S. A.","year":"1981","journal-title":"Cancer Res."},{"key":"e_1_2_7_31_1","first-page":"3675","article-title":"Curcumin inhibits cell proliferation by interfering with the cell cycle and inducing apoptosis in colon carcinoma cells","volume":"19","author":"Chen H.","year":"1999","journal-title":"Anticancer Res."},{"key":"e_1_2_7_32_1","first-page":"4334","article-title":"Histone deacetylase inhibitors decrease proliferation and modulate cell cycle gene expression in normal mammary epithelial cells","volume":"6","author":"Davis T.","year":"2000","journal-title":"Clin. Cancer Res."},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/23.4.581"},{"key":"e_1_2_7_34_1","first-page":"727","article-title":"Quercetin\u2010induced growth inhibition and cell death in nasopharyngeal carcinoma cells are associated with increase in Bad and hypophosphorylated retinoblastoma expressions","volume":"11","author":"Ong C. S.","year":"2004","journal-title":"Oncol. Rep."},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M312795200"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/bdrb.20236"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(78)90305-7"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2005.02.012"},{"key":"e_1_2_7_39_1","first-page":"523","article-title":"Short\u2010chain fatty acid\u2010initiated cell cycle arrest and apoptosis of colonic epithelial cells is linked to mitochondrial function","volume":"8","author":"Heerdt B. G.","year":"1997","journal-title":"Cell Growth Differ."},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgg.2012.05.008"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1021\/bp050228w"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coi.2008.06.007"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-1656(98)00044-3"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiotec.2014.05.024"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/15104"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiotec.2013.11.007"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.22812"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.21141"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.23075"}],"container-title":["Biotechnology Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbiot.201500327","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/biot.201500327","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T16:09:43Z","timestamp":1759939783000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/biot.201500327"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,12,29]]},"references-count":48,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2016,4]]}},"alternative-id":["10.1002\/biot.201500327"],"URL":"https:\/\/doi.org\/10.1002\/biot.201500327","archive":["Portico"],"relation":{},"ISSN":["1860-6768","1860-7314"],"issn-type":[{"value":"1860-6768","type":"print"},{"value":"1860-7314","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,12,29]]}}}