{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T02:27:37Z","timestamp":1772850457665,"version":"3.50.1"},"reference-count":39,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2016,5,4]],"date-time":"2016-05-04T00:00:00Z","timestamp":1462320000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["NSFC31271535 and NSFC31501235"],"award-info":[{"award-number":["NSFC31271535 and NSFC31501235"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007847","name":"Natural Science Foundation of Jilin Province","doi-asserted-by":"publisher","award":["JLSTF20130206013YY"],"award-info":[{"award-number":["JLSTF20130206013YY"]}],"id":[{"id":"10.13039\/100007847","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotechnology Journal"],"published-print":{"date-parts":[[2016,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Due to lack of commercial vaccine against the serogroup B (MenB) of <jats:italic>Neisseria meningitides<\/jats:italic>, the incidence of meningococcal disease remains high. To solve the issue, transgenic plants are used as bioreactors to produce a plant\u2010derived fHbp subunit vaccine. In this study, the fHbp gene was optimized according to the codon usage bias of <jats:italic>Arabidopsis thaliana<\/jats:italic>, synthesized artificially, cloned into an expression vector, driven by a seed\u2010specific promoter, and introduced into <jats:italic>A. thaliana<\/jats:italic> by <jats:italic>Agrobacterium<\/jats:italic>\u2010mediated floral\u2010dip transformation. Transgenic plants were identified by glufosinate selection, quickstix strips for PAT\/bar tests and PCR analysis. The five plants showing higher expression of recombinant fHbp were screened through indirect ELISA. Southern blot analysis showed that the transgenic line rHF\u201022 had a single\u2010copy integration and the highest expression of fHbp. Recombinant fHbp was purified from seeds of rHF\u201022 by nitrilotriacetic acid\u2010mediated affinity chromatography, and the purity was 82.5%. BALB\/c mice were tested for fHbp vaccine protection from lethal MenB infection, and the relative percent survival was found to be 80%. This study indicates that the recombinant fHbp produced from seeds of rHF\u201022 is a potential candidate for commercial MenB vaccine. It also provides a reference for safe, cheap and large\u2010scale production of other plant\u2010made vaccines.<\/jats:p>","DOI":"10.1002\/biot.201500656","type":"journal-article","created":{"date-parts":[[2016,4,27]],"date-time":"2016-04-27T18:45:56Z","timestamp":1461782756000},"page":"973-980","source":"Crossref","is-referenced-by-count":5,"title":["Over\u2010expression of fHbp in <i>Arabdopsis<\/i> for development of meningococcal serogroup B subunit vaccine"],"prefix":"10.1002","volume":"11","author":[{"given":"Nuo","family":"Xu","sequence":"first","affiliation":[{"name":"College of Life Sciences Jilin University  Changchun China"},{"name":"Wenzhou University  Wenzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yunpeng","family":"Wang","sequence":"additional","affiliation":[{"name":"Agro-Biotechnology Research Institute Jilin Academy of Agriculture Science  Changchun China"},{"name":"Wenzhou University  Wenzhou China"},{"name":"School of Pharmaceutical 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