{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,12]],"date-time":"2026-07-12T03:49:10Z","timestamp":1783828150459,"version":"3.55.0"},"reference-count":30,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,9,20]],"date-time":"2017-09-20T00:00:00Z","timestamp":1505865600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotechnology Journal"],"published-print":{"date-parts":[[2018,1]]},"abstract":"<jats:sec><jats:label\/><jats:p>The development of tubular engineered tissues is a challenging research area aiming to provide tissue substitutes but also in vitro models to test drugs, medical devices, and even to study physiological and pathological processes. In this work, the design, fabrication, and validation of an original cost\u2010effective tubular multilayered\u2010tissue culture system (TMCS) are reported. By exploiting cellularized collagen gel as scaffold, a simple moulding technique and an endothelialization step on a rotating system, TMCS allowed to easily prepare in 48\u2009h, trilayered arterial wall models with finely organized cellular composition and to mature them for 2 weeks without any need of manipulation. Multilayered constructs incorporating different combinations of vascular cells are compared in terms of cell organization and viscoelastic mechanical properties demonstrating that cells always progressively aligned parallel to the longitudinal direction. Also, fibroblast compacted less the collagen matrix and appeared crucial in term of maturation\/deposition of elastic extracellular matrix. Preliminary studies under shear stress stimulation upon connection with a flow bioreactor are successfully conducted without damaging the endothelial monolayer. Altogether, the TMCS herein developed, thanks to its versatility and multiple functionalities, holds great promise for vascular tissue engineering applications, but also for other tubular tissues such as trachea or oesophagus.<\/jats:p><\/jats:sec>","DOI":"10.1002\/biot.201700359","type":"journal-article","created":{"date-parts":[[2017,9,2]],"date-time":"2017-09-02T06:33:43Z","timestamp":1504334023000},"source":"Crossref","is-referenced-by-count":20,"title":["A Cost\u2010Effective Culture System for the In Vitro Assembly, Maturation, and Stimulation of Advanced Multilayered Multiculture Tubular Tissue Models"],"prefix":"10.1002","volume":"13","author":[{"given":"Caroline","family":"Loy","sequence":"first","affiliation":[{"name":"Laboratory for Biomaterials and Bioengineering Canada Research Chair I in Biomaterials and Bioengineering for the Innovation in Surgery Division of Regenerative Medicine Department of Min\u2010Met\u2010Materials Engineering Research Center of CHU de Qu\u00e9bec Laval University PLT\u20101745G Qu\u00e9bec City QC Canada G1V 0A6"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniele","family":"Pezzoli","sequence":"additional","affiliation":[{"name":"Laboratory for Biomaterials and Bioengineering Canada Research Chair I in Biomaterials and Bioengineering for the Innovation in Surgery Division of Regenerative Medicine Department of Min\u2010Met\u2010Materials Engineering Research Center of CHU de Qu\u00e9bec Laval University PLT\u20101745G Qu\u00e9bec City QC Canada G1V 0A6"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriele","family":"Candiani","sequence":"additional","affiliation":[{"name":"Department of Chemistry Materials and Chemical Engineering \u201cGiulio Natta\u201d Politecnico di Milano Milan Italy"},{"name":"The Protein Factory Research Center Politecnico of Milano and University of Insubria Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diego","family":"Mantovani","sequence":"additional","affiliation":[{"name":"Laboratory for Biomaterials and Bioengineering Canada Research Chair I in Biomaterials and Bioengineering for the Innovation in Surgery Division of Regenerative Medicine Department of Min\u2010Met\u2010Materials Engineering Research Center of CHU de Qu\u00e9bec Laval University PLT\u20101745G Qu\u00e9bec City QC Canada G1V 0A6"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,9,20]]},"reference":[{"key":"e_1_2_6_1_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.teb.2012.0305"},{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cardiores.2006.02.021"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2934816"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtcvs.2003.11.029"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0741-5214(93)90150-K"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/bip.20871"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/mabi.200600242"},{"key":"e_1_2_6_8_1","first-page":"e52812","volume":"0","author":"Meghezi S.","year":"2015","journal-title":"J. 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