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Recent studies have shown that Halo\u2010tag\u2010flanked recombinant human FGF7 can solve the problem of toxicity; however, its biological activity is unknown. This study aimed to explore the activity of Halo\u2010rhFGF7 and rhFGF7 on acute liver injury in vitro and in vivo. The rhFGF7 is expressed with a N\u2010terminal Halo\u2010tag, followed by a tobacco etch virus (TEV) protease cleavage site, in <jats:italic>Escherichia coli<\/jats:italic> BL21 (DE3) pLysS in this study. The products could stimulate the proliferation of carbon tetrachloride\u2013damaged L\u2010O<jats:sub>2<\/jats:sub> cells (normal human liver cells); they also inhibited cell apoptosis. Due to the use of the Halo, the protein could be tracked using fluorescence localization. Recombinant protein exerted a protective effect on the acute liver injury model in vitro and in vivo. The MTT assay and Western blot analysis showed that this protective effect is realized through various paths, including promoting proliferation, inhibiting cell apoptosis and anti\u2010inflammatory. In conclusion, Halo\u2010rhFGF7 and rhFGF7 displayed an excellent protective effect on acute liver injury. The present study provided an experimental basis and data support for further research on rhFGF7.<\/jats:p><\/jats:sec>","DOI":"10.1002\/biot.201700411","type":"journal-article","created":{"date-parts":[[2018,3,5]],"date-time":"2018-03-05T06:09:05Z","timestamp":1520230145000},"source":"Crossref","is-referenced-by-count":4,"title":["Effective Protection on Acute Liver Injury by Halo Tag\u2010Flanked Recombinant Fibroblast Growth Factor 7"],"prefix":"10.1002","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1238-9255","authenticated-orcid":false,"given":"Min","family":"Liu","sequence":"first","affiliation":[{"name":"School of Pharmaceutical Science, Key Laboratory of Biothechnology and Pharmaceutical Engineering of Zhejiang Province, Wenzhou Medical University Wenzhou Zhejiang 325035 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhiyu","family":"Chen","sequence":"additional","affiliation":[{"name":"The Second Affiliated Hospital, Wenzhou Medical University Wenzhou Zhejiang 325035 China"},{"name":"Quzhou Hospital of Traditional Chinese Medicine Quzhou Zhejiang 324000 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sisi","family":"Huang","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Science, Key Laboratory of Biothechnology and Pharmaceutical Engineering of Zhejiang Province, Wenzhou Medical University Wenzhou Zhejiang 325035 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shenghui","family":"Chu","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Science, Key Laboratory of Biothechnology and Pharmaceutical Engineering of Zhejiang Province, Wenzhou Medical University Wenzhou Zhejiang 325035 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nipatha","family":"Issaro","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Science, Key Laboratory of Biothechnology and Pharmaceutical Engineering of Zhejiang Province, Wenzhou Medical University Wenzhou Zhejiang 325035 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haishan","family":"Tian","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Science, Key Laboratory of Biothechnology and Pharmaceutical Engineering of Zhejiang Province, Wenzhou Medical University Wenzhou Zhejiang 325035 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haiyan","family":"Hu","sequence":"additional","affiliation":[{"name":"The Second Affiliated Hospital, Wenzhou Medical University Wenzhou Zhejiang 325035 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chao","family":"Jiang","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Science, Key Laboratory of Biothechnology and Pharmaceutical Engineering of Zhejiang Province, Wenzhou Medical University Wenzhou Zhejiang 325035 China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,5,17]]},"reference":[{"key":"e_1_2_8_1_1","first-page":"30","volume":"13","author":"Itoh N.","year":"2016","journal-title":"Front Cell Dev. 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