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Brasilicardin is a promising compound because of its unique mode of action and its higher potency and reduced toxicity compared to today's standard drugs. However, production of brasilicardin is so far hampered as <jats:italic>Nocardia terpenica<\/jats:italic> IFM 0406 synthesizes brasilicardin in only low amounts and represents a human pathogen (biosafety level 2 BSL2). In order to achieve a safe and high yield production of brasilicardin A (BraA), the authors heterologously express the brasilicardin gene cluster in the nocardioform actinomycete <jats:italic>Amycolatopsis japonicum<\/jats:italic> (<jats:italic>A. japonicum<\/jats:italic>::bcaAB01), which is fast growing, genetically accessible and closely related to <jats:italic>N. terpenica<\/jats:italic> IFM 0406. In <jats:italic>A. japonicum<\/jats:italic>::bcaAB01, four brasilicardin congeners, intermediates of the BraA biosynthesis, are produced. Investigation of the genes flanking the previously defined brasilicardin biosynthetic gene cluster revealed two novel genes (<jats:italic>bra0<\/jats:italic>, <jats:italic>bra12<\/jats:italic>), which are involved in brasilicardin biosynthesis: <jats:italic>bra12<\/jats:italic> encodes a transcriptional activator of the brasilicardin gene cluster. <jats:italic>bra0<\/jats:italic> codes for a dioxygenase involved in methoxylation of brasilicardin. 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