{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T15:14:56Z","timestamp":1792250096477,"version":"4.4.0"},"reference-count":54,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2018,2,5]],"date-time":"2018-02-05T00:00:00Z","timestamp":1517788800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP16K14881"],"award-info":[{"award-number":["JP16K14881"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP24246134"],"award-info":[{"award-number":["JP24246134"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotechnology Journal"],"published-print":{"date-parts":[[2018,5]]},"abstract":"<jats:sec><jats:label\/><jats:p><jats:italic>Corynebacterium glutamicum<\/jats:italic> is known for its ability to produce glutamic acid and has been utilized for the fermentative production of various amino acids. Glutamic acid production in <jats:italic>C. glutamicum<\/jats:italic> is induced by penicillin. In this study, the transcriptome and metabolome of <jats:italic>C. glutamicum<\/jats:italic> is analyzed to understand the mechanism of penicillin\u2010induced glutamic acid production. Transcriptomic analysis with DNA microarray revealed that expression of some glycolysis\u2010 and TCA cycle\u2010related genes, which include those encoding the enzymes involved in conversion of glucose to 2\u2010oxoglutaric acid, is upregulated after penicillin addition. Meanwhile, expression of some TCA cycle\u2010related genes, encoding the enzymes for conversion of 2\u2010oxoglutaric acid to oxaloacetic acid, and the anaplerotic reactions decreased. In addition, expression of NCgl1221 and <jats:italic>odhI<\/jats:italic>, encoding proteins involved in glutamic acid excretion and inhibition of the 2\u2010oxoglutarate dehydrogenase, respectively, is upregulated. Functional category enrichment analysis of genes upregulated and downregulated after penicillin addition revealed that genes for signal transduction systems are enriched among upregulated genes, whereas those for energy production and carbohydrate and amino acid metabolisms are enriched among the downregulated genes. As for the metabolomic analysis using capillary electrophoresis time\u2010of\u2010flight mass spectrometry, the intracellular content of most metabolites of the glycolysis and the TCA cycle decreased dramatically after penicillin addition. Overall, these results indicate that the cellular metabolism and glutamic acid excretion are mainly optimized at the transcription level during penicillin\u2010induced glutamic acid production by <jats:italic>C. glutamicum<\/jats:italic>.<\/jats:p><\/jats:sec>","DOI":"10.1002\/biot.201700612","type":"journal-article","created":{"date-parts":[[2018,1,11]],"date-time":"2018-01-11T07:42:46Z","timestamp":1515656566000},"source":"Crossref","is-referenced-by-count":31,"title":["Integrated Analysis of the Transcriptome and Metabolome of <i>Corynebacterium glutamicum<\/i> during Penicillin\u2010Induced Glutamic Acid Production"],"prefix":"10.1002","volume":"13","author":[{"given":"Takashi","family":"Hirasawa","sequence":"first","affiliation":[{"name":"School of Life Science and Technology Tokyo Institute of Technology 4259 Nagatsuta\u2010cho, Midori\u2010ku Yokohama Kanagawa 226\u20108501 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masaki","family":"Saito","sequence":"additional","affiliation":[{"name":"Department of Bioinformatic Engineering Graduate School of Information Science and Technology Osaka University 1\u20105 Yamadaoka Suita Osaka 565\u20100871 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katsunori","family":"Yoshikawa","sequence":"additional","affiliation":[{"name":"Department of Bioinformatic Engineering Graduate School of Information Science and Technology Osaka University 1\u20105 Yamadaoka Suita Osaka 565\u20100871 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chikara","family":"Furusawa","sequence":"additional","affiliation":[{"name":"Quantitative Biology Center RIKEN Suita Osaka 565\u20100874 Japan"},{"name":"Universal Biology Institute The University of Tokyo Tokyo 113\u20100033 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Shmizu","sequence":"additional","affiliation":[{"name":"Department of Bioinformatic Engineering Graduate School of Information Science and Technology Osaka University 1\u20105 Yamadaoka Suita Osaka 565\u20100871 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,2,5]]},"reference":[{"key":"e_1_2_7_1_1","doi-asserted-by":"publisher","DOI":"10.2323\/jgam.3.193"},{"key":"e_1_2_7_2_1","doi-asserted-by":"crossref","first-page":"754","DOI":"10.1128\/jb.79.5.754-755.1960","volume":"79","author":"Udaka S.","year":"1960","journal-title":"J. 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