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Although live\u2010attenuated vaccines are available for animals, they can only be used in endemic areas and there are currently no commercially available vaccines for humans. Here the authors describe the production of chimaeric RVFV virus\u2010like particles transiently expressed in <jats:italic>Nicotiana benthamiana<\/jats:italic> by <jats:italic>Agrobacterium tumefaciens\u2010<\/jats:italic>mediated gene transfer. The glycoprotein (<jats:italic>Gn<\/jats:italic>) gene is modified by removing its ectodomain (<jats:italic>Gne<\/jats:italic>) and fusing it to the transmembrane domain and cytosolic tail\u2010encoding region of avian influenza H5N1 hemagglutinin. This is expressed transiently in <jats:italic>N. benthamiana<\/jats:italic> with purified protein yields calculated to be \u224857\u2009mg\u2009kg<jats:sup>\u22121<\/jats:sup> fresh weight. Transmission electron microscopy shows putative chimaeric RVFV Gne\u2010HA particles of 49\u201360\u2009nm which are immunogenic, eliciting Gn\u2010specific antibody responses in vaccinated mice without the use of adjuvant. To our knowledge, this is the first demonstration of the synthesis of Gne\u2010HA chimaeric RVFV VLPs and the first demonstration of a detectable yield of RVFV Gn in plants.<\/jats:p><\/jats:sec>","DOI":"10.1002\/biot.201800238","type":"journal-article","created":{"date-parts":[[2018,11,29]],"date-time":"2018-11-29T02:37:43Z","timestamp":1543459063000},"source":"Crossref","is-referenced-by-count":16,"title":["Chimaeric Rift Valley Fever Virus\u2010Like Particle Vaccine Candidate Production in <i>Nicotiana benthamiana<\/i>"],"prefix":"10.1002","volume":"14","author":[{"given":"Sandiswa","family":"Mbewana","sequence":"first","affiliation":[{"name":"Biopharming Research Unit, Department of Molecular and Cell Biology, University of Cape Town Private Bag X3, 22 University Ave Rondebosch 7700 Cape Town South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2116-7913","authenticated-orcid":false,"given":"Ann E.","family":"Meyers","sequence":"additional","affiliation":[{"name":"Biopharming Research Unit, Department of Molecular and Cell Biology, University of Cape Town Private Bag X3, 22 University Ave Rondebosch 7700 Cape Town South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edward P.","family":"Rybicki","sequence":"additional","affiliation":[{"name":"Biopharming Research Unit, Department of Molecular and Cell Biology, University of Cape Town Private Bag X3, 22 University Ave Rondebosch 7700 Cape Town South Africa"},{"name":"Institute of Infectious Disease and Molecular Medicine, Faculty of Health Sciences, University of Cape Town Anzio Road Observatory 7925 South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,12,18]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF03401814"},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2214-109X(16)30176-0"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2009.07.046"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-3387-7_10"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2009.08.002"},{"key":"e_1_2_8_6_1","first-page":"127","volume":"365","author":"Hasler B.","year":"2013","journal-title":"Curr. 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