{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T19:07:07Z","timestamp":1776712027625,"version":"3.51.2"},"reference-count":27,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2018,9,25]],"date-time":"2018-09-25T00:00:00Z","timestamp":1537833600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotechnology Journal"],"published-print":{"date-parts":[[2019,4]]},"abstract":"<jats:sec><jats:label\/><jats:p>Capacitance is used to monitor \u201cbiomass\u201d in cell culture and fermentation processes. The present work explores various ways to support good manufacturing practices (GMP) use and create robust methods that can assure proper performance of capacitance equipment. An at\u2010line capacitance measurement method is particularly valuable as it can be used to correct or confirm online measurements from bioreactors. To obtain consistent predictions of online capacitance, the at\u2010line method requires appropriate control of culture temperature, vessel geometry, mixing, and timing. The at\u2010line method yields values that differ systematically from online values, but the variation is \u226411% for two cell lines tested. A cell line\u2010specific conversion factor addresses the difference and enables accurate predictions of online capacitance values. In addition to the at\u2010line method, verification of equipment performance is further enabled by using formaldehyde\u2010treated cell standards. The cell\u2010derived standards create a stabilized system for studying capacitance and recapitulate the performance of metabolically active cell culture. The formaldehyde\u2010treated cells, however, require a much greater conversion factor to predict online outputs. The stabilized system proves useful for evaluation and calibration of capacitance measuring systems. The at\u2010line method and stabilized cell\u2010derived suspensions support use of capacitance for process control in large scale mammalian cell culture.<\/jats:p><\/jats:sec>","DOI":"10.1002\/biot.201800283","type":"journal-article","created":{"date-parts":[[2018,9,10]],"date-time":"2018-09-10T05:30:47Z","timestamp":1536557447000},"source":"Crossref","is-referenced-by-count":13,"title":["Development of Capacitance Tools: At\u2010Line Method for Assessing Biomass of Mammalian Cell Culture and Fixed Cell Calibration Standard"],"prefix":"10.1002","volume":"14","author":[{"given":"Juhi","family":"Fernandes","sequence":"first","affiliation":[{"name":"Manufacturing Sciences Biogen 5000 Davis Dr. Research Triangle Park NC 27709"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jayme","family":"Currie","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering North Carolina State University Raleigh NC 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