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It is necessary to establish rapid detection method for SP. In this work, a monoclonal antibody (mAb)\u2010based gold immunochromatography assay (GICA) is developed for the rapid detection of SP. Under optimum conditions, the half\u2010maximal inhibitory concentration of SP\u2010mAb is 0.43\u00a0ng\u00a0mL<jats:sup>\u20131<\/jats:sup>. The subtype of SP\u2010mAb is IgG2b. This antibody has no cross\u2010reactivity with other analogues and has high affinity (4.52 \u00d7 10<jats:sup>10<\/jats:sup> L\u00a0mol<jats:sup>\u20131<\/jats:sup>). Qualitative results can be visualized with the naked eye, with a visual detection limit of 1.0\u00a0ng\u00a0mL<jats:sup>\u20131<\/jats:sup> and cut\u2010off value of 10\u00a0ng\u00a0mL<jats:sup>\u20131<\/jats:sup>. A hand\u2010held strip scanner is used for the quantitative analysis, with LOD 0.43\u00a0ng\u00a0mL<jats:sup>\u20131<\/jats:sup> in assay buffer. The recoveries of SP ranged from 72.3% to 112% in milk and 98.5% to 115% in beef, with variable coefficient ranging from 9.4% to 11.7% in milk and 8.14% to 15.4% in beef. Besides, the proposed GICA method for SP is confirmed by LC\u2013MS\/MS in SP\u2010spiked milk and beef samples. Overall, the developed GICA can be a useful tool for SP residues on\u2010site screening in milk and beef samples.<\/jats:p>","DOI":"10.1002\/biot.201900224","type":"journal-article","created":{"date-parts":[[2019,8,29]],"date-time":"2019-08-29T21:34:11Z","timestamp":1567114451000},"source":"Crossref","is-referenced-by-count":7,"title":["Gold Immunochromatography Assay for the Rapid Detection of Spiramycin in Milk and Beef Samples Based on a Monoclonal Antibody"],"prefix":"10.1002","volume":"15","author":[{"given":"Lingling","family":"Guo","sequence":"first","affiliation":[{"name":"State Key Laboratory of Food Science and Technology Jiangnan University  Wuxi 214122 P. R. 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