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Neutralizing antibodies against the EBOV surface glycoprotein (GP) can prevent infections, demonstrating a straightforward way for an efficient vaccination strategy. Meanwhile, many different anti\u2010EBOV antibodies have been identified, whereas the exact binding epitopes are often unknown. Here, the analysis of serum samples from an EBOV vaccine trial with the recombinant vesicular stomatitis virus\u2010Zaire ebolavirus (rVSV\u2010ZEBOV) and an Ebola virus disease survivor, using high\u2010density peptide arrays, is presented. In this proof\u2010of\u2010principle study, distinct IgG and IgM antibodies binding to different epitopes of EBOV GP is detected: By mapping the whole GP as overlapping peptide fragments, new epitopes and confirmed epitopes from the literature are found. Furthermore, the highly selective binding epitope of a neutralizing monoclonal anti\u2010EBOV GP antibody could be validated. This shows that peptide arrays can be a valuable tool to study the humoral immune response to vaccines in patients and to support Ebola vaccine development.<\/jats:p>","DOI":"10.1002\/biot.202000069","type":"journal-article","created":{"date-parts":[[2020,5,28]],"date-time":"2020-05-28T14:06:28Z","timestamp":1590674788000},"source":"Crossref","is-referenced-by-count":11,"title":["Epitopes of Naturally Acquired and Vaccine\u2010Induced Anti\u2010Ebola Virus Glycoprotein Antibodies in Single Amino Acid Resolution"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1537-6487","authenticated-orcid":false,"given":"Jasmin","family":"Heidepriem","sequence":"first","affiliation":[{"name":"Max Planck Institute of Colloids and Interfaces Department of Biomolecular Systems  Potsdam 14476 Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Verena","family":"Kr\u00e4hling","sequence":"additional","affiliation":[{"name":"Institute of Virology, Faculty of Medicine Philipps University Marburg  Marburg 35043 Germany"},{"name":"German Center for Infection Research partner site Gie\u00dfen\u2010Marburg\u2010Langen  Marburg 35043 Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christine","family":"Dahlke","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, First Department of Medicine University Medical Center Hamburg\u2010Eppendorf  Hamburg 20246 Germany"},{"name":"Department of Clinical Immunology of Infectious Diseases Bernhard Nocht Institute for Tropical Medicine  Hamburg 20359 Germany"},{"name":"German Center for Infection Research Partner Site Hamburg\u2010L\u00fcbeck\u2010Borstel\u2010Riems  Hamburg 20359 Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Timo","family":"Wolf","sequence":"additional","affiliation":[{"name":"Department of Medicine, Infectious Diseases Unit Goethe University Hospital  Frankfurt 60590 Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Florian","family":"Klein","sequence":"additional","affiliation":[{"name":"Laboratory of Experimental Immunology Institute of Virology University of Cologne Faculty of Medicine and University Hospital of Cologne  Cologne 50931 Germany"},{"name":"Center for Molecular Medicine Cologne (CMMC) University of Cologne Faculty of Medicine  Cologne 50931 Germany"},{"name":"German Center for Infection Research partner site Bonn\u2010Cologne  Cologne 50931 Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marylyn M.","family":"Addo","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, First Department of Medicine University Medical Center Hamburg\u2010Eppendorf  Hamburg 20246 Germany"},{"name":"Department of Clinical Immunology of Infectious Diseases Bernhard Nocht Institute for Tropical Medicine  Hamburg 20359 Germany"},{"name":"German Center for Infection Research Partner Site Hamburg\u2010L\u00fcbeck\u2010Borstel\u2010Riems  Hamburg 20359 Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephan","family":"Becker","sequence":"additional","affiliation":[{"name":"Institute of Virology, Faculty of Medicine Philipps University Marburg  Marburg 35043 Germany"},{"name":"German Center for Infection Research partner site Gie\u00dfen\u2010Marburg\u2010Langen  Marburg 35043 Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Felix F.","family":"Loeffler","sequence":"additional","affiliation":[{"name":"Max Planck Institute of Colloids and Interfaces Department of Biomolecular Systems  Potsdam 14476 Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,6,8]]},"reference":[{"key":"e_1_2_8_1_1","unstructured":"World Health Organization Ebola Virus Disease https:\/\/www.who.int\/news-room\/fact-sheets\/detail\/ebola-virus-disease(accessed: December 2019)."},{"key":"e_1_2_8_2_1","unstructured":"World Health Organization Ebola Virus Disease\u2013Democratic Republic of the Congo https:\/\/www.who.int\/csr\/don\/14-november-2019-ebola-drc\/en\/(accessed: December 2019)."},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1604330"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aad5224"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(19)30036-4"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiy285"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(18)30397-9"},{"key":"e_1_2_8_8_1","author":"Mulangu S.","year":"2019","journal-title":"N. 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