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Reasons are the need for an NAD(P)<jats:sup>+<\/jats:sup> cofactor regeneration system, often low performance in oxidative reactions and the limited substrate scope of ADHs. ADHA from <jats:italic>Candida magnoliae<\/jats:italic> DSMZ 70638 is identified to efficiently catalyze the regio\u2010selective hydroxy\u2010lactone oxidations to hydroxy\u2010lactones. Hydroxy\u2010lactones are common intermediates in industrial processes to cholesterol lowering (va)statin drugs. A biocatalytic aliphatic hydroxy\u2010lactone oxidation process is developed using pure oxygen as oxidant reaching volumetric productivities of up to 12\u00a0g L<jats:sup>\u22121<\/jats:sup> h<jats:sup>\u22121<\/jats:sup>, product concentrations of almost 50\u00a0g L<jats:sup>\u22121<\/jats:sup> and 95% reaction yield. For co\u2010factor recycling a previously engineered, water\u2010forming NAD(P)H\u2010oxidase from <jats:italic>Streptococcus mutans<\/jats:italic> is used. The process is scaled up to industrial pilot plant scale and it could be demonstrated that ADH catalyzed oxidations can be developed to efficient and safe processes. However, the ADHA wild\u2010type enzyme is not productive enough in chlorolactol oxidation. Therefore, enzyme engineering and multi\u2010parameter screening is successfully applied to optimize the enzyme for the target reaction. The optimized ADHA variant shows a 17\u2010fold higher oxidative activity, a 26\u00b0C increased stability and is applied to develop an efficient chlorolactol oxidation process.<\/jats:p>","DOI":"10.1002\/biot.202000171","type":"journal-article","created":{"date-parts":[[2020,9,1]],"date-time":"2020-09-01T03:15:14Z","timestamp":1598930114000},"source":"Crossref","is-referenced-by-count":23,"title":["Optimization of Alcohol Dehydrogenase for Industrial Scale Oxidation of Lactols"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4428-7501","authenticated-orcid":false,"given":"Sebastian","family":"Bartsch","sequence":"first","affiliation":[{"name":"c\u2010LEcta GmbH  Perlickstr. 5 04103 Leipzig Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Brummund","sequence":"additional","affiliation":[{"name":"InnoSyn B.V.  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