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This study presents the first description of WEEV virus\u2010like particles (VLPs) generated from insect cells using recombinant baculoviruses. WEEV VLPs with 206 adjuvant could trigger a strong cellular immune response; increase the levels of IL\u20102, IL\u20104 and IFN\u2010\u03b3; and induce a high level of neutralizing antibodies against WEEV in mice. These data showed that the insect cell\u2010baculovirus system is suitable for the production of WEEV VLPs and that these VLPs could elicit the strong immunogenicity in mice. These results suggest a new, nonreplicating, and effective vaccine candidate against WEEV infection.<\/jats:p>","DOI":"10.1002\/biot.202100008","type":"journal-article","created":{"date-parts":[[2021,6,27]],"date-time":"2021-06-27T04:49:16Z","timestamp":1624769356000},"source":"Crossref","is-referenced-by-count":2,"title":["Western equine encephalitis virus virus\u2010like particles from an insect cell\u2010baculovirus system elicit the strong immune responses in mice"],"prefix":"10.1002","volume":"16","author":[{"given":"JinZhu","family":"Ma","sequence":"first","affiliation":[{"name":"Key Laboratory of Jilin Province for Zoonosis Prevention and Control Changchun Veterinary Research Institute Chinese Academy of Agricultural Sciences  Changchun Jilin China"},{"name":"College of Life Science and Biotechnology Heilongjiang Bayi Agricultural University  Daqing Heilongjiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"HuaLei","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Jilin Province for Zoonosis Prevention and Control Changchun Veterinary Research Institute Chinese Academy of Agricultural Sciences  Changchun Jilin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"XueXing","family":"Zheng","sequence":"additional","affiliation":[{"name":"Key Laboratory of Jilin Province for Zoonosis Prevention and Control Changchun Veterinary Research Institute Chinese Academy of Agricultural Sciences  Changchun Jilin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"HongXia","family":"Wu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Jilin Province for Zoonosis Prevention and Control Changchun Veterinary Research Institute Chinese Academy of Agricultural Sciences  Changchun Jilin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0979-0526","authenticated-orcid":false,"given":"SongTao","family":"Yang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Jilin Province for Zoonosis Prevention and Control Changchun Veterinary Research Institute Chinese Academy of Agricultural Sciences  Changchun Jilin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"XianZhu","family":"Xia","sequence":"additional","affiliation":[{"name":"Key Laboratory of Jilin Province for Zoonosis Prevention and Control Changchun Veterinary Research Institute Chinese Academy of Agricultural Sciences  Changchun Jilin China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,7,9]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/MRA.01223-19"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2009.07.016"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2009.08.023"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1086\/444397"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.158.3806.1283"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1008102"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/v10040147"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.74.1913.227"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2010.09-0092"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0060427"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1177\/0300985810372508"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/v9070163"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00876-10"},{"key":"e_1_2_10_15_1","first-page":"151","article-title":"Complete genomic RNA sequence of western equine encephalitis virus and expression of the structural genes","volume":"81","author":"Netolitzky D. 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