{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T12:43:42Z","timestamp":1793105022355,"version":"4.5.0"},"reference-count":28,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2021,7,22]],"date-time":"2021-07-22T00:00:00Z","timestamp":1626912000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2017R1A2B3004972"],"award-info":[{"award-number":["2017R1A2B3004972"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["NRF\u20102020R1F1052024"],"award-info":[{"award-number":["NRF\u20102020R1F1052024"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002551","name":"Seoul National University","doi-asserted-by":"publisher","award":["#550\u20132020005"],"award-info":[{"award-number":["#550\u20132020005"]}],"id":[{"id":"10.13039\/501100002551","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotechnology Journal"],"published-print":{"date-parts":[[2022,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Many genome\u2010edited animals have been produced using clustered regularly interspaced short palindromic repeats (CRISPR)\u2010Cas9 technology to edit specific genes. However, there are few guidelines for the application of this technique to cattle. The goal of this study was to produce trait\u2010improved cattle using the genome\u2010editing technology CRISPR\u2013Cas9. Myostatin (\n                    <jats:italic>MSTN<\/jats:italic>\n                    ) was selected as a target locus, and synthetic mRNA of sgRNA and Cas9 were microinjected into fertilized bovine embryos in vitro. As a result, 17 healthy calves were born, and three of them showed\n                    <jats:italic>MSTN<\/jats:italic>\n                    mutation rates of 10.5%, 45.4%, and 99.9%, respectively. Importantly, the offspring with the 99.9%\n                    <jats:italic>MSTN<\/jats:italic>\n                    mutation rate had a biallelic mutation (\u201012\u00a0bps) and a double\u2010muscling phenotype. In conclusion, we demonstrate that the genome\u2010editing technology CRISPR\u2010Cas9 can produce genetically modified calves with improved traits.\n                  <\/jats:p>","DOI":"10.1002\/biot.202100198","type":"journal-article","created":{"date-parts":[[2021,7,11]],"date-time":"2021-07-11T04:46:20Z","timestamp":1625978780000},"source":"Crossref","is-referenced-by-count":61,"title":["Production of\n                    <i>MSTN<\/i>\n                    \u2010mutated cattle without exogenous gene integration using CRISPR\u2010Cas9"],"prefix":"10.1002","volume":"17","author":[{"given":"Gyeong\u2010Min","family":"Gim","sequence":"first","affiliation":[{"name":"Laboratory of Theriogenology and Biotechnology Department of Veterinary Clinical Science College of Veterinary Medicine and the Research Institute of Veterinary Science Seoul National University  Seoul Republic of Korea"},{"name":"BK21 Plus Program College of Veterinary Medicine Seoul National University  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong\u2010Hyeok","family":"Kwon","sequence":"additional","affiliation":[{"name":"Laboratory of Theriogenology and Biotechnology Department of Veterinary Clinical Science College of Veterinary Medicine and the Research Institute of Veterinary Science Seoul National University  Seoul Republic of Korea"},{"name":"BK21 Plus Program College of Veterinary Medicine Seoul National University  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyeong\u2010Hyun","family":"Eom","sequence":"additional","affiliation":[{"name":"Laboratory of Theriogenology and Biotechnology Department of Veterinary Clinical Science College of Veterinary Medicine and the Research Institute of Veterinary Science Seoul National University  Seoul Republic of Korea"},{"name":"BK21 Plus Program College of Veterinary Medicine Seoul National University  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"JoonHo","family":"Moon","sequence":"additional","affiliation":[{"name":"LARTBio Inc.  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji\u2010Hyun","family":"Park","sequence":"additional","affiliation":[{"name":"LARTBio Inc.  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Won\u2010Wu","family":"Lee","sequence":"additional","affiliation":[{"name":"LARTBio Inc.  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dae\u2010Jin","family":"Jung","sequence":"additional","affiliation":[{"name":"Gyeongsangbukdo Livestock Research Institute  Yeongju Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dae\u2010Hyun","family":"Kim","sequence":"additional","affiliation":[{"name":"Gyeongsangbukdo Livestock Research Institute  Yeongju Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun\u2010Koo","family":"Yi","sequence":"additional","affiliation":[{"name":"Gyeongsangbukdo Livestock Research Institute  Yeongju Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jae\u2010Jung","family":"Ha","sequence":"additional","affiliation":[{"name":"Gyeongsangbukdo Livestock Research Institute  Yeongju Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ka\u2010Yeong","family":"Lim","sequence":"additional","affiliation":[{"name":"Center for Genome Engineering Institute for Basic Science (IBS)  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin\u2010Soo","family":"Kim","sequence":"additional","affiliation":[{"name":"Center for Genome Engineering Institute for Basic Science (IBS)  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3804-1871","authenticated-orcid":false,"given":"Goo","family":"Jang","sequence":"additional","affiliation":[{"name":"Laboratory of Theriogenology and Biotechnology Department of Veterinary Clinical Science College of Veterinary Medicine and the Research Institute of Veterinary Science Seoul National University  Seoul Republic of Korea"},{"name":"BK21 Plus Program College of Veterinary Medicine Seoul National University  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,7,22]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gfs.2017.01.001"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11248-019-00141-6"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-817052-6.00007-0"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2009-2789"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.7.9.910"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.0030079"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng1810"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep14435"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa040933"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41576-018-0082-2"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.2013.3368"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.3560"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/cr.2011.153"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1271"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt783"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11248-016-9932-x"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40104-018-0232-6"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.15302\/J-FASE-2019305"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11248-014-9832-x"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btw561"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11103-014-0188-7"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.151270098"},{"key":"e_1_2_11_24_1","first-page":"251","article-title":"GDF\u20108 propeptide binds to GDF\u20108 and antagonizes biological activity by inhibiting GDF\u20108 receptor binding","volume":"18","author":"Tries R. 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