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The acetone biosynthesis pathway was constructed by combining genes from <jats:italic>Clostridium acetobutylicum<\/jats:italic> and <jats:italic>Clostridium aceticum<\/jats:italic>. Expression of acetone production genes was demonstrated in all strains. In bioreactors with continuous gas supply, all produced acetic acid, acetone, and, surprisingly, isopropanol. The production of isopropanol was caused by an endogenous secondary alcohol dehydrogenase (SADH) activity at low gas\u2010feeding rate. Although high amounts of the natural end product acetic acid of <jats:italic>A. woodii<\/jats:italic> were formed,14.5\u00a0mM isopropanol and 7.6\u00a0mM acetone were also detected, showing that this is a promising approach for the production of new solvents from C1 gases. The highest acetic acid, acetone, and isopropanol production was detected in the recombinant <jats:italic>A. woodii<\/jats:italic> [pJIR750_ac1t1] strain, with final concentrations of 438\u00a0mM acetic acid, 7.6\u00a0mM acetone, and 14.5\u00a0mM isopropanol. The engineered strain <jats:italic>A. woodii<\/jats:italic> [pJIR750_ac1t1] was found to be the most promising strain for acetone production from a gas mixture of CO<jats:sub>2<\/jats:sub> and H<jats:sub>2<\/jats:sub> and the formation of isopropanol in <jats:italic>A. woodii<\/jats:italic> was shown for the first time.<\/jats:p>","DOI":"10.1002\/biot.202100515","type":"journal-article","created":{"date-parts":[[2022,1,25]],"date-time":"2022-01-25T10:20:48Z","timestamp":1643106048000},"source":"Crossref","is-referenced-by-count":33,"title":["Engineering <i>Acetobacterium woodii<\/i> for the production of isopropanol and acetone from carbon dioxide and hydrogen"],"prefix":"10.1002","volume":"17","author":[{"given":"K\u00fcbra","family":"Arslan","sequence":"first","affiliation":[{"name":"Chemical Engineering Laboratory, Faculty of Sciences and Centre for Advanced Scientific Research (CICA), BIOENGIN group University of La Coru\u00f1a  La Coru\u00f1a Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teresa","family":"Schoch","sequence":"additional","affiliation":[{"name":"Institute of Microbiology and Biotechnology University of Ulm  Ulm Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franziska","family":"H\u00f6fele","sequence":"additional","affiliation":[{"name":"Institute of Microbiology and Biotechnology University of Ulm  Ulm Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabrina","family":"Herrschaft","sequence":"additional","affiliation":[{"name":"Institute of Microbiology and Biotechnology University of Ulm  Ulm Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catarina","family":"Oberlies","sequence":"additional","affiliation":[{"name":"Institute of Microbiology and Biotechnology University of Ulm  Ulm Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank","family":"Bengelsdorf","sequence":"additional","affiliation":[{"name":"Institute of Microbiology and Biotechnology University of Ulm  Ulm Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda C.","family":"Veiga","sequence":"additional","affiliation":[{"name":"Chemical Engineering Laboratory, Faculty of Sciences and Centre for Advanced Scientific Research (CICA), BIOENGIN group University of La Coru\u00f1a  La Coru\u00f1a Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"D\u00fcrre","sequence":"additional","affiliation":[{"name":"Institute of Microbiology and Biotechnology University of Ulm  Ulm Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3013-6713","authenticated-orcid":false,"given":"Christian","family":"Kennes","sequence":"additional","affiliation":[{"name":"Chemical Engineering Laboratory, Faculty of Sciences and Centre for Advanced Scientific Research (CICA), BIOENGIN group University of La Coru\u00f1a  La Coru\u00f1a Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,2,5]]},"reference":[{"key":"e_1_2_10_2_1","first-page":"176","volume-title":"Acetone. 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