{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T13:58:56Z","timestamp":1792159136412,"version":"4.4.0"},"reference-count":34,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2022,12,8]],"date-time":"2022-12-08T00:00:00Z","timestamp":1670457600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotechnology Journal"],"published-print":{"date-parts":[[2023,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Leptospirosis is a potentially life\u2010threatening zoonosis caused by pathogenic <jats:italic>Leptospira<\/jats:italic> and for rapid diagnostics, direct detection is desirable. LipL32 protein is the most suitable biomarker for direct detection. DNA aptamers are sought to be generated against LipL32 by Systemic Evolution of Ligands via Exponential Enrichment (SELEX). LepDapt\u20105a is the most potent aptamer candidate among all the candidates, as determined by direct Enzyme\u2010linked Aptasorbent Assay (ELASA). LepDapt\u20105a was predicted to form a G\u2010quadruplex structure as predicted by QGRS Mapper and validated experimentally by direct ELASA. The diagnostic potential of the aptamer was further tested on a direct and sandwich ELASA platform. A LOD of 10<jats:sup>6<\/jats:sup> mL<jats:sup>\u20101<\/jats:sup> and 10<jats:sup>5<\/jats:sup> mL<jats:sup>\u20101<\/jats:sup> were estimated by direct and sandwich ELASA platforms, respectively, which are within the range associated with leptospiremia levels. The dot blot assay developed was able to attain a LOD of 10<jats:sup>4<\/jats:sup> CFU mL<jats:sup>\u22121<\/jats:sup> against pathogenic <jats:italic>Leptospira<\/jats:italic>, which is also within the leptospiremia level. This is the first\u2010ever DNA aptamer and hybrid\u2010heterodimeric aptamer constructed against LipL32. The diagnostic potentiality of the LepDapt\u20105a DNA aptamer was proven on three major diagnostic platforms, which are direct ELASA, sandwich ELASA, and aptamer\u2010based dot assay.<\/jats:p>","DOI":"10.1002\/biot.202200418","type":"journal-article","created":{"date-parts":[[2022,11,25]],"date-time":"2022-11-25T05:09:30Z","timestamp":1669352970000},"source":"Crossref","is-referenced-by-count":15,"title":["Isolation of a novel DNA aptamer against LipL32 as a potential diagnostic agent for the detection of pathogenic <i>Leptospira<\/i>"],"prefix":"10.1002","volume":"18","author":[{"given":"Tzi Shien","family":"Yeoh","sequence":"first","affiliation":[{"name":"Advanced Medical &amp; Dental Institute (AMDI) Universiti Sains Malaysia  Bertam, 13200 Kepala Batas Penang Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thean\u2010Hock","family":"Tang","sequence":"additional","affiliation":[{"name":"Advanced Medical &amp; Dental Institute (AMDI) Universiti Sains Malaysia  Bertam, 13200 Kepala Batas Penang Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5395-0040","authenticated-orcid":false,"given":"Marimuthu","family":"Citartan","sequence":"additional","affiliation":[{"name":"Advanced Medical &amp; Dental Institute (AMDI) Universiti Sains Malaysia  Bertam, 13200 Kepala Batas Penang Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,12,8]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/346818a0"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2200121"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.287.5454.820"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkq615"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-5521(03)00028-0"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21124522"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/748913"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules25010003"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0003898"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0004122"},{"key":"e_1_2_9_12_1","volume-title":"Human leptospirosis : Guidance for diagnosis, surveillance and control","author":"World Health Organization","year":"2003"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.5.2414-2421.2003"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0051025"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/iai.64.6.2322-2330.1996"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1080\/07391102.2015.1009491"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/protein\/gzu006"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmii.2017.12.006"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12866-020-01744-4"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/1574-6968.12349"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1139\/w06-138"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11274-021-03209-w"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkg595"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkl253"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.3389\/fchem.2016.00038"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2009.02.038"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1080\/15321810701454870"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2021.113030"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkn517"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-7012(00)00224-4"},{"key":"e_1_2_9_31_1","first-page":"65","article-title":"Leptospirosis in humans","volume":"387","author":"Haake D. 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