{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T17:37:35Z","timestamp":1793036255550,"version":"4.5.0"},"reference-count":37,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2023,2,1]],"date-time":"2023-02-01T00:00:00Z","timestamp":1675209600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotechnology Journal"],"published-print":{"date-parts":[[2023,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Bone morphogenetic protein\u20102 (BMP\u20102) and transforming growth factor\u2010\u03b21 (TGF\u2010\u03b21) reportedly induce the osteogenic and tenogenic differentiation of anterior cruciate ligament (ACL)\u2010derived stem cells (LDSCs), respectively. However, few studies have investigated the effect of BMP\u20102\/TGF\u2010\u03b21 on the differentiation of LDSC. We developed a BMP\u20102\/TGF\u2010\u03b21 gene insertion into an LDSC cell sheet that promotes tendon\u2013bone healing in a mouse ACL reconstruction (ACLR) model.<\/jats:p>\n                  <jats:p>\n                    CD34\n                    <jats:sup>+<\/jats:sup>\n                    LDSCs were isolated from human ACL stump tissues, virally transduced to express BMP\u20102 or TGF\u2010\u03b21, and then embedded within cell sheets. All mice underwent ACLR using an autograft wrapped with a cell sheet and were randomly divided into three groups: BMP\u20102\u2010, TGF\u2010\u03b21\u2010, and BMP\u20102\/TGF\u2010\u03b21\u2010transduced. At 4 and 8\u00a0weeks, tendon\u2013bone healing was evaluated by micro\u2010CT, biomechanical test, and histological analysis. BMP\u20102 and TGF\u2010\u03b21 promoted the osteogenic and tenogenic differentiation of LDSC in vitro. BMP\u20102\/TGF\u2010\u03b21\u2010transduced LDSC sheet application contributed to early improvement in mean failure load and graft stiffness, accelerated maturation of the tendon\u2013bone junction, and inhibited bone tunnel widening. Furthermore, reduced M1 macrophage infiltration and a higher M2 macrophage percentage were observed in the BMP\u20102\/TGF\u2010\u03b21\u2010transduced LDSC group.\n                  <\/jats:p>\n                  <jats:p>\n                    This work demonstrated that BMP\u20102 and TGF\u2010\u03b21 promoted CD34\n                    <jats:sup>+<\/jats:sup>\n                    LDSCs osteogenic and tenogenic differentiation in vitro and in vivo, which accelerated the tendon\u2013bone healing after ACLR using autografts wrapped with cell sheets in a mouse model.\n                  <\/jats:p>","DOI":"10.1002\/biot.202200470","type":"journal-article","created":{"date-parts":[[2023,1,23]],"date-time":"2023-01-23T03:43:59Z","timestamp":1674445439000},"source":"Crossref","is-referenced-by-count":20,"title":["BMP\u20102\/TGF\u2010\u03b21 gene insertion into ligament\u2010derived stem cells sheet promotes tendon\u2013bone healing in a mouse"],"prefix":"10.1002","volume":"18","author":[{"given":"Bing","family":"Wei","sequence":"first","affiliation":[{"name":"School of Medicine Southeast University  Nanjing Jiangsu Province China"},{"name":"Department of Orthopaedic Surgery\/Joint and Sports Medicine Center Zhongda Hospital Southeast University  Nanjing Jiangsu Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhuang","family":"Li","sequence":"additional","affiliation":[{"name":"School of Medicine Southeast University  Nanjing Jiangsu Province China"},{"name":"Department of Orthopaedic Surgery\/Joint and Sports Medicine Center Zhongda Hospital Southeast University  Nanjing Jiangsu Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yucheng","family":"Lin","sequence":"additional","affiliation":[{"name":"School of Medicine Southeast University  Nanjing Jiangsu Province China"},{"name":"Department of Orthopaedic Surgery\/Joint and Sports Medicine Center Zhongda Hospital Southeast University  Nanjing Jiangsu Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyue","family":"Hu","sequence":"additional","affiliation":[{"name":"School of Medicine Southeast University  Nanjing Jiangsu Province China"},{"name":"Department of Orthopaedic Surgery\/Joint and Sports Medicine Center Zhongda Hospital Southeast University  Nanjing Jiangsu Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Xu","sequence":"additional","affiliation":[{"name":"School of Medicine Southeast University  Nanjing Jiangsu Province China"},{"name":"Department of Orthopaedic Surgery\/Joint and Sports Medicine Center Zhongda Hospital Southeast University  Nanjing Jiangsu Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shanzheng","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Medicine Southeast University  Nanjing Jiangsu Province China"},{"name":"Department of Orthopaedic Surgery\/Joint and Sports Medicine Center Zhongda Hospital Southeast University  Nanjing Jiangsu Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingliang","family":"Ji","sequence":"additional","affiliation":[{"name":"School of Medicine Southeast University  Nanjing Jiangsu Province China"},{"name":"Department of Orthopaedic Surgery\/Joint and Sports Medicine Center Zhongda Hospital Southeast University  Nanjing Jiangsu Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9361-6087","authenticated-orcid":false,"given":"Jun","family":"Lu","sequence":"additional","affiliation":[{"name":"School of Medicine Southeast University  Nanjing Jiangsu Province China"},{"name":"Department of Orthopaedic Surgery\/Joint and Sports Medicine Center Zhongda Hospital Southeast University  Nanjing Jiangsu Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,2]]},"reference":[{"issue":"4","key":"e_1_2_11_2_1","first-page":"133","article-title":"[Radical removal of carotid chemodectoma followed by prosthetic repair of the internal carotid artery]","volume":"14","author":"Cherniavskii A. 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