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Wild\u2010type\n                    <jats:italic>Yarrowia lipolytica<\/jats:italic>\n                    IMUFRJ 50682 can be a biocatalyst to biodepolymerize PET. Herein, we report its ability to perform oxidative biotransformation of EG into glycolic acid (GA): a higher value\u2010added chemical with varied industrial applications. We found that this yeast tolerates high EG concentrations (up to 2\u00a0M) based on maximum non\u2010inhibitory concentration (MNIC) tests. Whole\u2010cell biotransformation assays using resting yeast cells showed GA production uncoupled to cell growth metabolism, and\n                    <jats:sup>13<\/jats:sup>\n                    C nuclear magnetic resonance (NMR) analysis confirmed GA production. Moreover, higher agitation speed (450 vs. 350\u00a0rpm) resulted in a 1.12\u2010fold GA production improvement (from 352 to 429.5\u00a0mM) during\n                    <jats:italic>Y. lipolytica<\/jats:italic>\n                    cultivation in bioreactors after 72\u00a0h. GA was constantly accumulated in the medium, suggesting that this yeast may also share an incomplete oxidation pathway (i.e., it is not metabolized to carbon dioxide) as seen in acetic acid bacterial group. Additional assays using higher chain\u2010length diols (1,3\u2010propanediol, 1,4\u2010butanediol, and 1,6\u2010hexanediol) revealed that C4 and C6 diols were more cytotoxic, suggesting that they underwent different pathways in the cells. We found that this yeast consumed extensively all these diols, however,\n                    <jats:sup>13<\/jats:sup>\n                    C NMR analysis from supernatant identified solely the presence of 4\u2010hydroxybutanoic acid from 1,4\u2010butanediol, along with GA from EG oxidation. Findings reported herein reveal a potential route for PET upcycling to a higher value\u2010added product.\n                  <\/jats:p>","DOI":"10.1002\/biot.202200521","type":"journal-article","created":{"date-parts":[[2023,3,10]],"date-time":"2023-03-10T05:30:24Z","timestamp":1678426224000},"source":"Crossref","is-referenced-by-count":40,"title":["Biotransformation of ethylene glycol to glycolic acid by\n                    <i>Yarrowia lipolytica<\/i>\n                    : A route for poly(ethylene terephthalate) (PET) upcycling"],"prefix":"10.1002","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9675-4813","authenticated-orcid":false,"given":"Adriano","family":"Carniel","sequence":"first","affiliation":[{"name":"Department of Biochemical Engineering Escola de Qu\u00edmica, Universidade Federal do Rio de Janeiro (UFRJ) \u2013 Cidade Universit\u00e1ria  Rio de Janeiro RJ Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7803-9827","authenticated-orcid":false,"given":"Ariane Gaspar","family":"Santos","sequence":"additional","affiliation":[{"name":"Department of Biochemical Engineering Escola de Qu\u00edmica, Universidade Federal do Rio de Janeiro (UFRJ) \u2013 Cidade Universit\u00e1ria  Rio de Janeiro RJ Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0974-0465","authenticated-orcid":false,"suffix":"J\u00fanior","given":"Luiz Silvino","family":"Chinelatto","sequence":"additional","affiliation":[{"name":"Petrobras Research Development and Innovation Center (Cenpes) sAv. 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