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However, if recognized as a \u201cforeign body,\u201d insertion of a barrier membrane in GBR treatment will inevitably alter the local immune microenvironment and subsequently influence bone regeneration. The aim of this investigation was to fabricate decellularized periosteum (DP) and investigate its immunomodulatory properties in GBR. DP was successfully fabricated from periosteum from the mini\u2010pig cranium. In vitro experiments indicated that the DP scaffold modulated macrophage polarization toward a pro\u2010regenerative M2 phenotype, which in turn facilitated migration and osteogenic differentiation of bone marrow\u2010derived mesenchymal stem cells. A rat GBR model with a cranial critical\u2010size defect was established, and our in vivo experiment confirmed the beneficial effects of DP on the local immune microenvironment and bone regeneration. Collectively, the findings of this study indicate that the prepared DP possesses immunomodulatory properties and represents a promising barrier membrane for GBR procedures.<\/jats:p>","DOI":"10.1002\/biot.202300094","type":"journal-article","created":{"date-parts":[[2023,6,10]],"date-time":"2023-06-10T09:33:10Z","timestamp":1686389590000},"source":"Crossref","is-referenced-by-count":8,"title":["Decellularized periosteum promotes guided bone regeneration via manipulation of macrophage polarization"],"prefix":"10.1002","volume":"18","author":[{"given":"Jiayang","family":"Li","sequence":"first","affiliation":[{"name":"Department of Oral and Maxillofacial\u2010Head and Neck Oncology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine; College of Stomatology, Shanghai Jiao Tong University; National Center for Stomatology; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology; Shanghai Research Institute of Stomatology Shanghai Center of Head and Neck Oncology Clinical and Translational Science Shanghai China"},{"name":"Department of Endodontics, Shanghai Stomatological Hospital, Fudan University; 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