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One of the challenges in studying the mechanism of acne is that current in vitro culture methods cannot reflect the 3D cellular environment in the tissue, including inflammatory stimuli and cellular interactions especially the interactions between sebocytes and immune cells. To solve this issue, we generated an in vitro acne disease model consisting of 3D artificial sebocyte glands and macrophages through the inertial focusing effect method. Using this model, we produced a controllable inflammatory environment similar to the acne pathogenetic process in the skin. The 3D artificial sebocyte glands and macrophages can be separated for analyzing each cell type, assisting the in\u2010depth understanding of the acne mechanism. This study indicates that proinflammatory macrophages promote lipid accumulation and induce oxidative stress in sebocyte glands. Additionally, in an inflammatory environment, sebocyte glands induce macrophage polarization into the M1 phenotype. Employing this model for drug screening, we also demonstrated that, cannabidiol (CBD), a clinically investigated drug, is effective in restoring lipid accumulation, oxidative stress, inflammatory cytokines and macrophage polarization in the acne disease.<\/jats:p>","DOI":"10.1002\/biot.202300108","type":"journal-article","created":{"date-parts":[[2023,7,21]],"date-time":"2023-07-21T10:59:39Z","timestamp":1689937179000},"source":"Crossref","is-referenced-by-count":6,"title":["In vitro acne disease model from inertial focusing effect for studying the interactions between sebocyte glands and macrophages"],"prefix":"10.1002","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9652-8659","authenticated-orcid":false,"given":"Tan","family":"Tang","sequence":"first","affiliation":[{"name":"Department of Material Processing and Controlling School of Mechanical Engineering &amp; Automation Beihang University Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ye","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Material Processing and Controlling School of Mechanical Engineering &amp; Automation Beihang University Beijing China"},{"name":"Center of Soft Matter Physics and Its Applications Beihang University Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lelin","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Material Processing and Controlling School of Mechanical Engineering &amp; Automation Beihang University Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peipei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Material Processing and Controlling School of Mechanical Engineering &amp; Automation Beihang University Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,8,3]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/19381980.2017.1375636"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(11)60321-8"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12724285"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2013.505"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0625.2009.00890.x"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/iai.63.8.3158-3165.1995"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2009.11.017"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.4161\/derm.1.2.8736"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clindermatol.2004.03.004"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjd.15754"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjd.15879"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.imbio.2011.01.001"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dsi.2015.12.004"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/jdv.13190"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.5582\/ddt.2018.01079"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/exd.14184"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-0716-0648-3_5"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-10261-6"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2013.07.129"},{"key":"e_1_2_9_21_1","first-page":"53","article-title":"Challenges in bio\u2010fabrication of organoid cultures","volume":"1107","author":"Peng W. 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