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To achieve this goal, in this work, a practical impedimetric biosensor has been implemented into a disposable electrode on a screen\u2010printed carbon electrode (SPCE) for the detection of two mosquito\u2010borne viruses. The biosensor fabrication has step\u2010wisely carried out on the disposable electrode surface at room temperature: starting from conductive film formation, physical binding of the gold nanoparticles (AuNPs)\u2010polyaniline (PAni) into the conductive film, and biofunctionalization. To get the maximum efficiency of the antibody, biotinylated antibody has been conjugated on the surface of AuNP\u2010PAni\/PAni\u2010SPCE via the streptavidin\u2010biotin conjugation method which is a critical factor for the high sensitivity. Using the antibody\u2013antigen interaction, this disposable electrode has designed to detect mosquito\u2010borne infectious viruses, Chikungunya virus (CHIKV), and Zika virus (ZIKV) separately in a wide linear range of 100\u00a0fg\u00a0mL<jats:sup>\u22121<\/jats:sup> to 1\u00a0ng\u00a0mL<jats:sup>\u22121<\/jats:sup> with a low detection limit of 1.33 and 12.31\u00a0fg\u00a0mL<jats:sup>\u22121<\/jats:sup>, respectively.<\/jats:p>","DOI":"10.1002\/biot.202300125","type":"journal-article","created":{"date-parts":[[2023,5,2]],"date-time":"2023-05-02T00:47:40Z","timestamp":1682988460000},"source":"Crossref","is-referenced-by-count":8,"title":["Development of disposable electrode for the detection of mosquito\u2010borne viruses"],"prefix":"10.1002","volume":"18","author":[{"given":"Fahmida","family":"Nasrin","sequence":"first","affiliation":[{"name":"Laboratory of Biotechnology, Research Institute of Green Science and Technology Shizuoka University  Suruga\u2010ku Shizuoka 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