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The kit performs RNA extraction, reverse transcriptase loop\u2010mediated isothermal amplification (RT\u2010LAMP), and visual detection in one kit. An acrylic stick was engraved with one shallow and one deep cylindrical chamber at each end for the insertion of an FTA card and ethidium homodimer\u20101 (EthD\u20101), respectively, to perform RNA extraction\/purification and bimodal visual detection of the target amplicons. First, an intercalation of EthD\u20101 into the target DNA initially produces fluorescence upon UV illumination. Next, the addition of a strong oxidant, in this case sodium (meta) periodate (NaIO<jats:sub>4<\/jats:sub>), produces intense aggregates in the presence of EthD\u20101\u2010intercalated DNA, realized by electrostatic interaction. In the absence of the target amplicon, no fluorescence or aggregates are observed. Using this kit, two major infectious viruses\u2014severe fever with thrombocytopenia syndrome virus (SFTSV) and severe acute respiratory syndrome coronavirus (SARS\u2010CoV\u20102)\u2014were successfully detected in 1\u00a0h, and the limits of detection (LOD) were approximately 1 virus \u03bcL<jats:sup>\u22121<\/jats:sup> for SFTSV and 10<jats:sup>3<\/jats:sup> copies \u03bcL<jats:sup>\u22121<\/jats:sup> for SARS\u2010CoV\u20102 RNA. The introduced kit is portable, end\u2010user\u2010friendly, and can be operated in a pipette\u2010free manner, paving the way for simple and convenient virus detection in resource\u2010limited settings.<\/jats:p>","DOI":"10.1002\/biot.202300521","type":"journal-article","created":{"date-parts":[[2024,2,25]],"date-time":"2024-02-25T21:00:36Z","timestamp":1708894836000},"source":"Crossref","is-referenced-by-count":5,"title":["Pipette\u2010free field\u2010deployable molecular diagnostic kit for bimodal visual detection of infectious RNA viruses"],"prefix":"10.1002","volume":"19","author":[{"given":"So Yeon","family":"Park","sequence":"first","affiliation":[{"name":"Department of BioNano Technology Gachon University  Seongnam\u2010si Gyeonggi\u2010do Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5318-4742","authenticated-orcid":false,"given":"Kieu The 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