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We developed a hepatic patch by combining decellularized liver matrix (DLM) with the hepatocyte growth factor (HGF)\/heparin\u2013complex and evaluated its restorative efficacy. In vitro prophylactic results, the HGF\/heparin\u2013DLM patches effectively mitigated CCl<jats:sub>4<\/jats:sub>\u2010induced hepatocyte toxicity and restored the cytotoxicity levels to the baseline levels by day 5. Furthermore, these patches restored albumin synthesis of injured hepatocytes to more than 70% of the normal levels within 5 days. In vitro therapeutic results, the urea synthesis of the injured hepatocytes reached 91% of the normal levels after 10 days of culture, indicating successful restoration of hepatic function by the HGF\/heparin\u2013DLM patches in both prophylactic and therapeutic models. In vivo results, HGF\/heparin\u2013DLM patches attached to the liver and gut exhibited a significant decrease in collagen content (4.44 times and 2.77 times, respectively) and an increase in glycogen content (1.19 times and 1.12 times, respectively) compared to the fibrosis group after 1\u00a0week, separately. In summary, liver function was restored and inflammation was inhibited through the combined effects of DLM and the HGF\/heparin\u2013complex in fibrotic liver. The newly designed hepatic patch holds promise for both in vitro and in vivo regeneration therapy and preventive health care for liver tissue engineering.<\/jats:p>","DOI":"10.1002\/biot.202300570","type":"journal-article","created":{"date-parts":[[2024,6,12]],"date-time":"2024-06-12T06:49:14Z","timestamp":1718174954000},"source":"Crossref","is-referenced-by-count":4,"title":["Fabrication of a decellularized liver matrix\u2013based hepatic patch for the repair of CCl4\u2010induced liver injury"],"prefix":"10.1002","volume":"19","author":[{"given":"Ting\u2010Yi","family":"Wu","sequence":"first","affiliation":[{"name":"Department of Biomechatronics Engineering National Taiwan University  Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi\u2010Cheng","family":"Hsieh","sequence":"additional","affiliation":[{"name":"Department of Biomechatronics Engineering National Taiwan University  Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei\u2010Rong","family":"Yin","sequence":"additional","affiliation":[{"name":"Department of Biomechatronics Engineering National Taiwan University  Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai\u2010Yi","family":"Cheng","sequence":"additional","affiliation":[{"name":"Department of Biomechatronics Engineering National Taiwan University  Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4978-9829","authenticated-orcid":false,"given":"Yung\u2010Te","family":"Hou","sequence":"additional","affiliation":[{"name":"Department of Biomechatronics Engineering National Taiwan University  Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,6,12]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.b.34506"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(21)01374-X"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2020.2298"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.820290807"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1177\/2041731420986711"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep4.1136"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3002376"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2020.09.014"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsbiomaterials.9b00745"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1089\/scd.2019.0130"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2021.120899"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.reth.2018.07.001"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2016.06.015"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2020.06.016"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12253-011-9430-7"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.2147\/TCRM.S115902"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bej.2022.108354"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/785474"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiosc.2010.01.016"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/mabi.202300411"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bej.2022.108757"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep17567"},{"key":"e_1_2_11_24_1","unstructured":"Lala V. 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