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However, most studies published to date used fetal bovine serum (FBS) as culture medium supplement for SH\u2010SY5Y cell differentiation. We report on the testing of human platelet lysate (hPL) as a culture medium supplement to support SH\u2010SY5Y cell culture. Both standard hPL and a fibrinogen\u2010depleted hPL (FD\u2010hPL) formulation, which does not require the addition of anticoagulants to culture media, promoted an increase in SH\u2010SY5Y cell proliferation in comparison to FBS, without compromising metabolic activity. SH\u2010SY5Y cells cultured in hPL or FD\u2010hPL also displayed a higher number of neurite extensions and stained positive for MAP2 and synaptophysin, in the absence of differentiation stimuli; reducing hPL or FD\u2010hPL concentration to 1% v\/v did not affect cell proliferation or metabolic activity. Furthermore, following treatment with retinoic acid (RA) and further stimulation with brain\u2010derived neurotrophic factor (BDNF) and nerve growth factor beta (NGF\u2010\u03b2), the percentage of SH\u2010SY5Y cells stained positive for dopaminergic neuronal differentiation markers (tyrosine hydroxylase [TH] and Dopamine Transporter [DAT]) was higher in hPL or FD\u2010hPL than in FBS, and gene expression of dopaminergic markers TH, DAT, and DR2 was also detected.<\/jats:p><jats:p>Overall, the data herein presented supports the use of hPL to differentiate SH\u2010SY5Y cells into a neuronal phenotype with dopaminergic features, and the adoption of FD\u2010hPL as a fully xenogeneic free alternative to FBS to support the use of SH\u2010SY5Y cells as a neurodegeneration model.<\/jats:p>","DOI":"10.1002\/biot.202400068","type":"journal-article","created":{"date-parts":[[2024,7,10]],"date-time":"2024-07-10T22:15:04Z","timestamp":1720649704000},"source":"Crossref","is-referenced-by-count":2,"title":["Human platelet lysate supports SH\u2010SY5Y neuroblastoma cell proliferation and differentiation into a dopaminergic\u2010like neuronal phenotype under xenogeneic\u2010free culture conditions"],"prefix":"10.1002","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7903-2974","authenticated-orcid":false,"given":"Miguel","family":"Ribeiro","sequence":"first","affiliation":[{"name":"Life and Health Sciences Research Institute (ICVS) Universidade do Minho  Braga Portugal"},{"name":"ICVS\/3B's\u2013\u2013PT Government Associate Laboratory, Braga\/  Guimar\u00e3es Braga Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonas","family":"Campos","sequence":"additional","affiliation":[{"name":"Life and Health Sciences Research Institute (ICVS) Universidade do Minho  Braga Portugal"},{"name":"ICVS\/3B's\u2013\u2013PT Government Associate Laboratory, Braga\/  Guimar\u00e3es Braga Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiffany S.","family":"Pinho","sequence":"additional","affiliation":[{"name":"Life and Health Sciences Research Institute (ICVS) Universidade do Minho  Braga Portugal"},{"name":"ICVS\/3B's\u2013\u2013PT Government Associate Laboratory, Braga\/  Guimar\u00e3es Braga Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bel\u00e9m","family":"Sampaio\u2010Marques","sequence":"additional","affiliation":[{"name":"Life and Health Sciences Research Institute (ICVS) Universidade do Minho  Braga Portugal"},{"name":"ICVS\/3B's\u2013\u2013PT Government Associate Laboratory, Braga\/  Guimar\u00e3es Braga 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