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However, the reliance of 1,2\u2010PDO production in <jats:italic>Escherichia coli<\/jats:italic> on anaerobic conditions, as enhancing cell growth to augment precursor availability remains a substantial challenge. This study presents glucose\u2010based aerobic production of 1,2\u2010PDO, with xylose utilization facilitating cell growth. An engineered strain was constructed capable of exclusively producing 1,2\u2010PDO from glucose while utilizing xylose to support cell growth. This was accomplished by deleting the <jats:italic>gloA<\/jats:italic>, <jats:italic>eno<\/jats:italic>, <jats:italic>eda<\/jats:italic>, <jats:italic>sdaA<\/jats:italic>, <jats:italic>sdaB<\/jats:italic>, and <jats:italic>tdcG<\/jats:italic> genes for 1,2\u2010PDO production from glucose and introducing the Weimberg pathway for cell growth using xylose. Enhanced 1,2\u2010PDO production was achieved via <jats:italic>yagF<\/jats:italic> overexpression and disruption of the <jats:italic>ghrA<\/jats:italic> gene involved in the 1,2\u2010PDO\u2010competing pathway. The resultant strain, PD72, produced 2.48\u00a0\u00b1\u00a00.15\u00a0g\u00a0L<jats:sup>\u22121<\/jats:sup> 1,2\u2010PDO with a 0.27\u00a0\u00b1\u00a00.02\u00a0g\u00a0g<jats:sup>\u22121<\/jats:sup>\u2010glucose yield after 72\u00a0h cultivation. Overall, this study demonstrates aerobic 1,2\u2010PDO synthesis through the isolation of the 1,2\u2010PDO synthetic pathway from the tricarboxylic acid cycle.<\/jats:p>","DOI":"10.1002\/biot.202400210","type":"journal-article","created":{"date-parts":[[2024,8,21]],"date-time":"2024-08-21T22:36:09Z","timestamp":1724279769000},"source":"Crossref","is-referenced-by-count":7,"title":["Parallel metabolic pathway engineering for aerobic 1,2\u2010propanediol production in <i>Escherichia coli<\/i>"],"prefix":"10.1002","volume":"19","author":[{"given":"Daisuke","family":"Nonaka","sequence":"first","affiliation":[{"name":"Department of Chemical Science and Engineering, Graduate School of Engineering Kobe University, Nada\u2010ku  Kobe Hyogo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuuki","family":"Hirata","sequence":"additional","affiliation":[{"name":"Department of Chemical Science and Engineering, Graduate School of Engineering Kobe University, Nada\u2010ku  Kobe 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