{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T02:11:41Z","timestamp":1776132701447,"version":"3.50.1"},"reference-count":34,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2024,10,9]],"date-time":"2024-10-09T00:00:00Z","timestamp":1728432000000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22278187"],"award-info":[{"award-number":["22278187"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotechnology Journal"],"published-print":{"date-parts":[[2024,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The hydrolysis of cellulose generates inhibitors like acetate, suppressing fermentation performance. Here, 25SrRNA methyltransferase CgBMT5 from stress\u2010tolerant yeast <jats:italic>Candida glycerinogenes<\/jats:italic> was used as an anti\u2010stress gene element in <jats:italic>Saccharomyces cerevisiae<\/jats:italic> and <jats:italic>Yarrowia lipolytica<\/jats:italic>. Expression of <jats:italic>CgBMT5<\/jats:italic> in <jats:italic>S. cerevisiae<\/jats:italic> increased cell tolerance to acetate, high osmolarity, and heat stress and rescued the delay in cell growth under acetate stress. Ethanol productivity was improved from 0.52 g\u00b7(L\/h) to 0.69 g\u00b7(L\/h). CgBMT5 improved GFP expression. The transcription factor ARG81 binds to the promoter of <jats:italic>CgBMT5<\/jats:italic>. CgBMT5 upregulated <jats:italic>HOG1<\/jats:italic>, <jats:italic>GPD1<\/jats:italic>, <jats:italic>HAA1<\/jats:italic>, and <jats:italic>PMA1<\/jats:italic> and reduced ROS level, thereby improving cell resistance to acetate. CgBMT5 also improved resistance of <jats:italic>Y. lipolytica<\/jats:italic> Po1g to multiple\u2010stress. The lipid titer was improved by 37% in the typical medium. <jats:italic>Y. lipolytica\u2010CgBMT5<\/jats:italic> produced 94\u00a0mg\/L lipid in the undetoxified cellulose hydrolysate.<\/jats:p>","DOI":"10.1002\/biot.202400397","type":"journal-article","created":{"date-parts":[[2024,10,9]],"date-time":"2024-10-09T03:52:45Z","timestamp":1728445965000},"source":"Crossref","is-referenced-by-count":4,"title":["25SrRNA Methyltransferase CgBMT5 From <i>Candida glycerinogenes<\/i> Improves Tolerance and Fermentation Performance of <i>Saccharomyces cerevisiae<\/i> and <i>Yarrowia lipolytica<\/i> From Undetoxified Cellulose Hydrolysate"],"prefix":"10.1002","volume":"19","author":[{"given":"Xinyao","family":"Lu","sequence":"first","affiliation":[{"name":"The Key Laboratory of Industrial Biotechnology Ministry of Education School of Biotechnology Jiangnan University  Wuxi China"},{"name":"Lab of Industrial Microorganism &amp; Research and Design Center for Polyols School of Biotechnology Jiangnan University  Wuxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-7114-9913","authenticated-orcid":false,"given":"Xiaoqing","family":"Hao","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Industrial Biotechnology Ministry of Education School of Biotechnology Jiangnan University  Wuxi China"},{"name":"Lab of Industrial Microorganism &amp; 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