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Integrating various NPs can further enhance these effects. Herein, the zinc oxide (ZnO)\u2010MXene\u2010Ag nanocomposite was prepared, and the conjugation of its three components was confirmed through scanning electron microscopy (SEM) and energy dispersive X\u2010ray (EDX) mapping analysis. In vitro analysis using the agar well diffusion technique demonstrated that ZnO\u2010MXene\u2010Ag nanocomposite exhibited high antimicrobial efficacy, significantly inhibiting <jats:italic>Escherichia coli<\/jats:italic>, <jats:italic>Salmonella<\/jats:italic>, and <jats:italic>Candida albicans<\/jats:italic>, and showing enhanced potency when combined with tetracycline, resulting in a 2.6\u2010fold increase against <jats:italic>Staphylococcus<\/jats:italic> and a 2.4\u2010fold increase against <jats:italic>Pseudomonas<\/jats:italic>. The efficacy of nanocomposite\u2010loaded carboxymethyl cellulose (CMC) gel on wound healing was investigated using varying concentrations (0, 1, 5, and 10\u00a0mg\/mL). Wound healing was monitored over 21 days, with results indicating that wounds treated with 1\u00a0mg\/mL ZnO\u2010MXene\u2010Ag gel exhibited superior healing compared to the control group (0\u00a0mg\/mL), with significant improvements noted from Day 3 onward. Conversely, higher concentrations (10\u00a0mg\/mL) resulted in reduced healing efficiency, particularly notable on Day 15. In conclusion, the ZnO\u2010MXene\u2010Ag nanocomposite\u2010loaded CMC gel is a promising agent for enhanced wound healing and antimicrobial applications. These findings highlight the importance of optimizing NP concentration to maximize therapeutic benefits while minimizing potential cytotoxicity.<\/jats:p>","DOI":"10.1002\/biot.202400448","type":"journal-article","created":{"date-parts":[[2024,10,9]],"date-time":"2024-10-09T03:53:11Z","timestamp":1728445991000},"source":"Crossref","is-referenced-by-count":5,"title":["Concentration\u2010Dependent Effects of MXene Nanocomposite\u2010Loaded Carboxymethyl Cellulose on Wound Healing"],"prefix":"10.1002","volume":"19","author":[{"given":"Salma","family":"Nasser","sequence":"first","affiliation":[{"name":"Department of Surgery Faculty of Veterinary Medicine Alexandria University  Alexandria Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed Abd","family":"Elkodous","sequence":"additional","affiliation":[{"name":"Department of Electrical and Electronic Information Engineering Toyohashi University of Technology  Toyohashi Aichi 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