{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T16:33:13Z","timestamp":1792081993851,"version":"4.4.0"},"reference-count":41,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,2,9]],"date-time":"2025-02-09T00:00:00Z","timestamp":1739059200000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR\u201018\u2010EURE\u20100021"],"award-info":[{"award-number":["ANR\u201018\u2010EURE\u20100021"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotechnology Journal"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>This study conceptualizes the design of a small\u2010scale system (250\u00a0mL\u20131\u00a0L) for the autotrophic cultivation of hydrogen\u2010oxidizing bacteria, such as the representative strain <jats:italic>Cupriavidus necator<\/jats:italic>. The research aimed to systematically investigate the impact of bottle volume and gas composition, particularly oxygen concentration, on the growth and performance of <jats:italic>C. necator<\/jats:italic> during autotrophic cultivations. To this end, customized, pressure\u2010tight, baffled glass bottles of various sizes (250, 500, and 1000\u00a0mL) and gas mixtures with varying oxygen concentrations (4%, 8%, and 12% v\/v) were tested. Growth was monitored by measuring optical density. The maximum specific growth rate (<jats:italic>\u00b5<\/jats:italic><jats:sub>max<\/jats:sub>), the biomass production rate (BPR), the volumetric gas\u2013liquid mass transfer coefficient (<jats:italic>k<jats:sub>L<\/jats:sub>a<\/jats:italic>), and the oxygen transfer rate were calculated. Among the various combinations, the 1000\u2010mL bottles demonstrated the highest <jats:italic>\u00b5<\/jats:italic><jats:sub>max<\/jats:sub> (0.13 h<jats:sup>\u22121<\/jats:sup>) and the second\u2010highest BPR (0.074 g\u00a0L<jats:sup>\u22121\u00a0<\/jats:sup>h<jats:sup>\u22121<\/jats:sup>) at an oxygen concentration of 8%, without the need to refill the headspace. The proposed small\u2010scale system offers a swift and replicable method for concurrently investigating multiple autotrophic cultivations. In this regard, increasing the size of the bottle flask proved to be an efficient strategy to minimize the periodicity for gas refilling. Due to the inhibitory effect of oxygen, changing the liquid\u2013gas volume ratio in hydrogen\u2010driven shake flask cultivation had so far strongly influenced the growth rate. Our results provide a solid foundation for the scaling and optimization of small\u2010scale cultivation of chemolithotrophic bacteria and will facilitate future parallelization and, hence, optimization of metabolic aspects.<\/jats:p>","DOI":"10.1002\/biot.202400641","type":"journal-article","created":{"date-parts":[[2025,2,11]],"date-time":"2025-02-11T05:20:34Z","timestamp":1739251234000},"source":"Crossref","is-referenced-by-count":7,"title":["Conceptual Approach for Aerobic Autotrophic Gas Cultivation in Shake Flasks: Overcoming the Inhibitory Effects of Oxygen in <i>Cupriavidus necator<\/i>"],"prefix":"10.1002","volume":"20","author":[{"given":"Federico","family":"Di Bisceglie","sequence":"first","affiliation":[{"name":"Toulouse Biotechnology Institute (TBI) Universit\u00e9 de Toulouse, CNRS, INRAE, INSA  Toulouse France"},{"name":"Institute of Biotechnology and Biochemical Engineering Graz University of Technology  Graz Austria"},{"name":"Austrian Centre of Industrial Biotechnology 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