{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T15:09:41Z","timestamp":1790953781189,"version":"4.1.0"},"reference-count":50,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2025,7,17]],"date-time":"2025-07-17T00:00:00Z","timestamp":1752710400000},"content-version":"vor","delay-in-days":16,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002923","name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas","doi-asserted-by":"publisher","award":["PIBAA\u2010CONICET 2022"],"award-info":[{"award-number":["PIBAA\u2010CONICET 2022"]}],"id":[{"id":"10.13039\/501100002923","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotechnology Journal"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Bacterial vaccines using recombinant antigens displayed on the bacterial surface represent a major advancement in vaccine development. This approach leverages bacteria's natural ability to induce immune responses while improving vaccine targeting and efficacy. Existing platforms, such as live bacterial vaccines, inactivated bacterial vaccines, and bacterial\u2010like particles (BLPs), face challenges like genetic modifications, antigen dilution, and degradation. To address these limitations, a novel system called coated bacterial vaccine (CBV) is proposed, combining the properties of inactivated bacterial vaccines and BLPs technology. The recombinant antigen is fused to the carboxy\u2010terminal cell wall\u2010binding domain of the\n                    <jats:italic>Lactobacillus<\/jats:italic>\n                    SlpA protein (dSLPA) and anchored in vitro on chemically inactivated Gram\u2010positive bacteria. To validate this system, CBVs were created using inactivated\n                    <jats:italic>B. subtilis<\/jats:italic>\n                    15245 and the TTFC antigen, a fragment of tetanus toxin (TeTx) fused with dSLPA. CBVs were used to immunize BALB\/c mice, and anti\u2010TTFC IgG antibodies were measured. Mice were then challenged with TeTx to assess the protective effects of CBVs. Results showed a 100% survival rate in vaccinated mice after TeTx challenge, driven by a robust anti\u2010TTFC IgG response, particularly elevated IgG1 levels. Additionally, macrophage stimulation assays showed increased mRNA levels for IL\u20101\u03b2, IL\u20106, and IL\u201010, along with significant IL\u20106 secretion. These findings demonstrate that CBVs based on B. subtilis can promote active immune protection against TeTx challenge, likely mediated by a Th2\u2010polarized immune response, highlighting the potential of CBVs as a versatile tool for developing new\u2010generation vaccines.\n                  <\/jats:p>","DOI":"10.1002\/biot.70015","type":"journal-article","created":{"date-parts":[[2025,7,18]],"date-time":"2025-07-18T06:59:23Z","timestamp":1752821963000},"source":"Crossref","is-referenced-by-count":8,"title":["Coated Bacterial Vaccine: A New Approach for Antigen Display on Bacterial Surface"],"prefix":"10.1002","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-1617-0918","authenticated-orcid":false,"given":"In\u00e9s","family":"Harguindeguy","sequence":"first","affiliation":[{"name":"Center for Research and Development of Industrial Fermentations (CINDEFI CONICET\u2010UNLP)  La Plata Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-4038-7224","authenticated-orcid":false,"given":"Mat\u00edas H.","family":"Assandri","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology Department of Biological Sciences Faculty of Exact Sciences National University of La Plata (UNLP)  La Plata Buenos Aires Argentina"},{"name":"Institute of Health Sciences Arturo Jauretche National University (UNAJ)  Florencio Varela Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6412-1774","authenticated-orcid":false,"given":"Juan P.","family":"Gorgojo","sequence":"additional","affiliation":[{"name":"Center for Research and Development of Industrial Fermentations (CINDEFI CONICET\u2010UNLP)  La Plata Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6647-4677","authenticated-orcid":false,"given":"Yanina","family":"Hiriart","sequence":"additional","affiliation":[{"name":"Institute of Immunological and Pathophysiological Studies (IIFP\u2010UNLP\u2010CONICET)  La Plata Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanina","family":"Alarc\u00f3n","sequence":"additional","affiliation":[{"name":"Biological Institute Dr. Tom\u00e1s Per\u00f3n, Ministry of Health of the Province of Buenos Aires  La Plata Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8652-9172","authenticated-orcid":false,"given":"Mar\u00eda A.","family":"Huergo","sequence":"additional","affiliation":[{"name":"Institute of Theoretical and Applied Physical\u2010Chemical Research UNLP\u2010CONICET  La Plata Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0878-3586","authenticated-orcid":false,"given":"Sebasti\u00e1n F.","family":"Cavalitto","sequence":"additional","affiliation":[{"name":"Center for Research and Development of Industrial Fermentations (CINDEFI CONICET\u2010UNLP)  La Plata Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3960-080X","authenticated-orcid":false,"given":"Mar\u00eda de los A.","family":"Serradell","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology Department of Biological Sciences Faculty of Exact Sciences National University of La Plata (UNLP)  La Plata Buenos Aires Argentina"},{"name":"Institute of Health Sciences Arturo Jauretche National University (UNAJ)  Florencio Varela Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5073-3355","authenticated-orcid":false,"given":"Gast\u00f3n E.","family":"Ortiz","sequence":"additional","affiliation":[{"name":"Center for Research and Development of Industrial Fermentations (CINDEFI CONICET\u2010UNLP)  La Plata Buenos Aires Argentina"},{"name":"Biotechnology Institute National University of Hurlingham  Hurlingham Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,7,17]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.4103\/0974\u2010777X.77299"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41577\u2010020\u201000479\u20107"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.4103\/JFMPC.JFMPC_596_23"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/VACCINES11101600"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/1475\u20102859\u20105\u201023"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41541\u2010022\u201000503\u2010y"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom13060947"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/VACCINES3040940"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.imlet.2018.03.006"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/iai.67.9.4847\u20104850.1999"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-801238-3.00141-0"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/S12934\u2010016\u20100468\u20109"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2023.1263586"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2013.00282"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.3390\/vaccines11081292"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2023.1082273"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.509380"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12866\u2010022\u201002608\u20109"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1080\/21655979.2015.1040956"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms10051038"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2011.04.083"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2023.1126533"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13273\u2010022\u201000221\u20105"},{"key":"e_1_2_10_25_1","volume-title":"Molecular Cloning: A Laboratory Manual","author":"Sambrook J.","year":"2001"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978\u20100\u201012\u2010420070\u20108.00011\u20108"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2089"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1186\/2193\u20101801\u20102\u201089"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2023.1210898"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2017.04.004"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1093\/ilar.46.3.269"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01668\u201020"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.3390\/covid3010003"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.3390\/vaccines10071149"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1186\/1475\u20102859\u20106\u20101"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471\u20102199\u201011\u201090"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.02553\u201014"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pep.2013.10.016"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiotec.2004.08.004"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/biot.201100025"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.3390\/vaccines11030695"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.177.10.2684\u20102694.1995"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.6.1758\u20101768.2004"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00356\u201009"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253\u2010019\u201009795\u2010y"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11274\u2010020\u201002945\u20109"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1586\/14760584.2015.1051469"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1601641"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0901569"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12602\u2010019\u201009612\u2010y"}],"container-title":["Biotechnology Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/biot.70015","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,29]],"date-time":"2025-11-29T12:41:26Z","timestamp":1764420086000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/biot.70015"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7]]},"references-count":50,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2025,7]]}},"alternative-id":["10.1002\/biot.70015"],"URL":"https:\/\/doi.org\/10.1002\/biot.70015","archive":["Portico"],"relation":{"has-preprint":[{"id-type":"doi","id":"10.22541\/au.173425326.64330236\/v1","asserted-by":"object"}]},"ISSN":["1860-6768","1860-7314"],"issn-type":[{"value":"1860-6768","type":"print"},{"value":"1860-7314","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7]]},"article-number":"e70015"}}