{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T10:03:18Z","timestamp":1789034598203,"version":"build-2803163510"},"reference-count":26,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,4,25]],"date-time":"2025-04-25T00:00:00Z","timestamp":1745539200000},"content-version":"vor","delay-in-days":24,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotechnology Journal"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>L\u2010valine, an essential branched\u2010chain amino acid, is widely used across various industrial sectors. Despite its significance, there is a scarcity of continuous fermentation methodologies, specifically for L\u2010valine production. Biofilm\u2010based Technologies face challenges in sustaining continuous L\u2010valine production due to cell wall damage caused by L\u2010valine sterilization. Here, we combined continuous fermentation principles with Biofilm\u2010based technologies to develop a biofilm\u2010based continuous fermentation (BCF) system, enabling sustained L\u2010valine production. By analyzing the fermentation kinetics of free\u2010cell fermentation (FCF), we optimized the L\u2010valine titer in BCF to 60\u201370\u00a0g\/L, achieving a yield of 0.44\u00a0g\/g glucose\u2014a 10% increase over FCF. Notably, reducing the controlled L\u2010valine titer in BCF extended the fermentation time but simultaneously decreased both the yield and production rate. Conversely, shortening the fermentation time was associated with an increase in both yield and production rate. In summary, BCF significantly improved L\u2010valine purity, yield from glucose, and production rate while reducing by\u2010product formation.<\/jats:p>","DOI":"10.1002\/biot.70027","type":"journal-article","created":{"date-parts":[[2025,4,26]],"date-time":"2025-04-26T02:27:27Z","timestamp":1745634447000},"source":"Crossref","is-referenced-by-count":8,"title":["Integrating Biofilm\u2010Based Fermentation With Continuous Processes for Improved L\u2010Valine Production"],"prefix":"10.1002","volume":"20","author":[{"given":"Caice","family":"Liang","sequence":"first","affiliation":[{"name":"National Engineering Research Center for Biotechnology College of Biotechnology and Pharmaceutical Engineering Nanjing Tech University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Wang","sequence":"additional","affiliation":[{"name":"National Engineering Research Center for Biotechnology College of Biotechnology and Pharmaceutical Engineering Nanjing Tech University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuqi","family":"Shi","sequence":"additional","affiliation":[{"name":"National Engineering Research Center for Biotechnology College of Biotechnology and Pharmaceutical Engineering Nanjing Tech University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alan","family":"Yan","sequence":"additional","affiliation":[{"name":"Dulwich College  Singapore Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingguo","family":"Liu","sequence":"additional","affiliation":[{"name":"National Engineering Research Center for Biotechnology College of Biotechnology and Pharmaceutical Engineering Nanjing Tech University  Nanjing China"},{"name":"Nanjing Hi\u2010Tech Biological Technology Research Institute Co. 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