{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T20:58:33Z","timestamp":1781038713041,"version":"3.54.1"},"reference-count":48,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2025,7,4]],"date-time":"2025-07-04T00:00:00Z","timestamp":1751587200000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotechnology Journal"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Mammalian cell culture technologies are crucial for recombinant protein production, organoid generation, medical applications, and the generation of in vitro cultivated meat. However, they are limited by high costs, lack of vascular O\n                    <jats:sub>2<\/jats:sub>\n                    \u2010provision, and the resultant inhibition of 3D tissue formation. Effective media and nutrient usage, oxygenation, and waste management are\u00a0key to improvement. Microalgae utilize organic or inorganic CO\n                    <jats:sub>2<\/jats:sub>\n                    to produce O\n                    <jats:sub>2<\/jats:sub>\n                    from light, which complements O\n                    <jats:sub>2<\/jats:sub>\n                    \u2010consuming and CO\n                    <jats:sub>2<\/jats:sub>\n                    \u2010respiring mammalian cells in culture. However, common microalgal cultivation conditions differ in temperature and salinity from mammalian cell cultivation environments, making co\u2010cultivation short\u2010lived and challenging. We screened several different microalgae species to identify\n                    <jats:italic>Chlorella<\/jats:italic>\n                    sp. BDH\u20101 (BDH\u20101), which has high growth rates in mammalian culture conditions, but unlike other\n                    <jats:italic>Chlorella<\/jats:italic>\n                    species, does not compete for glucose as an energy source. In co\u2010culture, BDH\u20101 reduces cellular waste production by maintaining mammalian cells in oxidative phosphorylation, which stabilizes pH, tripling culture longevity, and optimizes nutrient usage, which increases growth performance up to 80%. It further allows the reduction of expensive and ethically challenging fetal bovine serum requirements. Collectively, mammalian cell\/BDH\u20101 co\u2010cultivation improves tissue culture health and reduces costs, paving the path for applications in the biotechnology and medical sectors.\n                  <\/jats:p>","DOI":"10.1002\/biot.70067","type":"journal-article","created":{"date-parts":[[2025,7,4]],"date-time":"2025-07-04T03:30:38Z","timestamp":1751599838000},"source":"Crossref","is-referenced-by-count":5,"title":["Co\u2010Cultivation With New Glucose\u2010Sparing\n                    <i>Chlorella<\/i>\n                    Algae Boosts Tissue Culture Efficiency by Reducing Cell Waste"],"prefix":"10.1002","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9290-0580","authenticated-orcid":false,"given":"Melanie","family":"Oey","sequence":"first","affiliation":[{"name":"Institute for Molecular Bioscience The University of Queensland  Brisbane Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ute","family":"Marx","sequence":"additional","affiliation":[{"name":"Pforzheim University  Pforzheim Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Horst Joachim","family":"Schirra","sequence":"additional","affiliation":[{"name":"School of Environment and Science Griffith University  Brisbane Australia"},{"name":"Institute for Biomedicine and Glycomics Griffith University  Brisbane Australia"},{"name":"Australian Institute for Bioengineering and Nanotechnology The University of Queensland  Brisbane Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2389-4292","authenticated-orcid":false,"given":"Ian L.","family":"Ross","sequence":"additional","affiliation":[{"name":"Institute for Molecular Bioscience The University of Queensland  Brisbane Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert G.","family":"Parton","sequence":"additional","affiliation":[{"name":"Institute for Molecular Bioscience The University of Queensland  Brisbane Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ben","family":"Hankamer","sequence":"additional","affiliation":[{"name":"Institute for Molecular Bioscience The University of Queensland  Brisbane Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harriet P.","family":"Lo","sequence":"additional","affiliation":[{"name":"Institute for Molecular Bioscience The University of Queensland  Brisbane Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,7,4]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-811710-1.00012-4"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/hsr2.449"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.5483\/BMBRep.2022-0195"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioadv.2023.213428"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2021.e07686"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1525-1594.2006.00289.x"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.2006.12.337"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep41594"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.algal.2020.102008"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13068\u2010022\u201002249\u20100"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/1873-3468.13070"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10397-008-0464-0"},{"key":"e_1_2_10_14_1","volume-title":"Field Guide to Appropriate Technology","author":"Hazeltine B.","year":"2003"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.12372"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.psep.2020.10.006"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13068\u2010022\u201002249\u20100"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jwpe.2021.102382"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40899\u2010023\u201000887\u20102"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1515\/znb-1963-0905"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.algal.2015.07.005"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.3390\/foods10102318"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.3390\/foods10102318"},{"key":"e_1_2_10_24_1","unstructured":"E. 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