{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,13]],"date-time":"2026-06-13T03:50:01Z","timestamp":1781322601416,"version":"3.54.1"},"reference-count":43,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2025,7,4]],"date-time":"2025-07-04T00:00:00Z","timestamp":1751587200000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotechnology Journal"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Thermoregulatory systems are rarely utilized in the synthetic biology of\n                    <jats:italic>Streptomyces<\/jats:italic>\n                    . In\n                    <jats:italic>Streptomyces hygroscopicus<\/jats:italic>\n                    TL01, the previous finding of enhanced validamycin biosynthesis at 37\u00b0C indicated the involvement of a thermoregulatory system. In this study, the\n                    <jats:italic>\n                      P\n                      <jats:sub>valA<\/jats:sub>\n                    <\/jats:italic>\n                    (\n                    <jats:italic>\n                      P\n                      <jats:sub>KA<\/jats:sub>\n                    <\/jats:italic>\n                    ) promoter was thermoregulated in\n                    <jats:italic>Streptomyces albus<\/jats:italic>\n                    J1074, and its regulation was determined by a GC\u2010rich DNA clamp.\n                    <jats:italic>\n                      P\n                      <jats:sub>KA<\/jats:sub>\n                    <\/jats:italic>\n                    \u2010binding regulatory proteins were identified through DNA affinity chromatography. Overexpression of\n                    <jats:italic>SHJG_5852<\/jats:italic>\n                    and\n                    <jats:italic>SHJG_8253<\/jats:italic>\n                    in\n                    <jats:italic>S. albus<\/jats:italic>\n                    resulted in the\n                    <jats:italic>\n                      P\n                      <jats:sub>KA<\/jats:sub>\n                    <\/jats:italic>\n                    strength ratios of 2.61 and 4.34 at 37\u00b0C and 30\u00b0C, respectively. In contrast, the overexpression of SHJG_6392 led to an inhibited transcription of the\n                    <jats:italic>\n                      P\n                      <jats:sub>KA<\/jats:sub>\n                    <\/jats:italic>\n                    at 30\u00b0C. A thermoregulatory system was reconstituted in\n                    <jats:italic>S. albus<\/jats:italic>\n                    J1074 with a shorter but more efficient promoter derived from the\n                    <jats:italic>\n                      P\n                      <jats:sub>KA<\/jats:sub>\n                    <\/jats:italic>\n                    ,\n                    <jats:italic>SHJG_5852<\/jats:italic>\n                    , and\n                    <jats:italic>SHJG_6392<\/jats:italic>\n                    . With this system, the induced indigoidine titer at 37\u00b0C was 3.56 times that at 30\u00b0C. In summary, we identified thermoregulatory factors involved in validamycin biosynthesis and reconstituted a system for the thermoregulated production of actinobacterial natural products.\n                  <\/jats:p>","DOI":"10.1002\/biot.70068","type":"journal-article","created":{"date-parts":[[2025,7,4]],"date-time":"2025-07-04T03:34:58Z","timestamp":1751600098000},"source":"Crossref","is-referenced-by-count":3,"title":["Identification of Thermoregulatory Factors Involved in Validamycin Biosynthesis and Reconstitution of a Thermoregulated Expression System"],"prefix":"10.1002","volume":"20","author":[{"given":"Yudian","family":"Yu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Microbial Metabolism School of Life Sciences &amp; Biotechnology Shanghai Jiao Tong University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qianjin","family":"Kang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Microbial 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