{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T06:06:32Z","timestamp":1773900392038,"version":"3.50.1"},"reference-count":43,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2025,8,10]],"date-time":"2025-08-10T00:00:00Z","timestamp":1754784000000},"content-version":"vor","delay-in-days":9,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotechnology Journal"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>3D printing is emerging as a promising fabrication technique for microfluidic devices. In this work, this technology was exploited in the development of a microfluidic chromatographic column with nominal volume of 54\u00a0\u00b5L. The microcolumn was packed with a cation exchange resin and characterized, using potassium iodide as a tracer, in terms of porosity (<jats:italic>\u03b5<\/jats:italic>\u00a0=\u00a00.72), plate number, and asymmetry factor (0.8\u00a0&lt;\u00a0A<jats:sub>S<\/jats:sub>\u00a0&lt;\u00a01.8 for flowrates &gt;50\u00a0\u00b5L\/min). To showcase the potential of this microdevice, it was exploited in the characterization of the chromatographic behavior of lysozyme. The measured saturation capacity (<jats:italic>q<\/jats:italic><jats:sup>\u221e<\/jats:sup>=\u00a088.14\u00a0g\/L<jats:sub>resin<\/jats:sub> at 340\u00a0cm\/h) was in line with the manufacturer declaration (85\u2013135\u00a0g\/L at &lt;500\u00a0cm\/h). In addition, the effect of NaCl at different concentrations on the protein adsorption isotherm was characterized, demonstrating a Langmuir to anti\u2010Langmuir transition at concentrations \u2265300\u00a0mM. The axial dispersion coefficient was finally determined (= 6.7 \u00b7 10<jats:sup>\u22129<\/jats:sup> m<jats:sup>2<\/jats:sup>\/s). In this way, the mcirofluidic column\u00a0allowed to develop a comprehensive mechanistic model describing the transport of lysozyme in the chromatographic medium using only 30\u00a0\u00b5L of resin and &lt;1\u00a0g of protein, addressing the issue of limited availability of biomolecules and streamlining the process development.<\/jats:p>","DOI":"10.1002\/biot.70095","type":"journal-article","created":{"date-parts":[[2025,8,11]],"date-time":"2025-08-11T05:55:01Z","timestamp":1754891701000},"source":"Crossref","is-referenced-by-count":2,"title":["3D Printed Microfluidic Chromatographic Column for Fast Downstream Processing Development"],"prefix":"10.1002","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-1146-0813","authenticated-orcid":false,"given":"Vladimir","family":"Matining","sequence":"first","affiliation":[{"name":"Department of Chemistry Materials and Chemical Engineering \u201cGiulio Natta\u201d Politecnico Di Milano, Piazza Leonardo Da Vinci 32  Milan 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