{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T17:53:18Z","timestamp":1790445198810,"version":"4.1.0"},"reference-count":62,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2025,9,15]],"date-time":"2025-09-15T00:00:00Z","timestamp":1757894400000},"content-version":"vor","delay-in-days":14,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004503","name":"Benha University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004503","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotechnology Journal"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Alginates and kappa\u2010carrageenan (KC) are natural anionic biopolymers widely utilized in food, pharmaceutical, and cosmetic industries for their anticoagulant, immunomodulatory, and antiviral properties. However, unmodified sodium alginate\/KC (SA\/KC) hydrogels often lack sufficient antimicrobial and antioxidant efficacy, limiting their functionality in advanced biomedical and packaging applications. To overcome these limitations, we designed a multifunctional hydrogel incorporating silver nanoparticles (AgNPs) into an ascorbic acid\u2010crosslinked SA\/KC matrix. Comprehensive physicochemical, morphological, antibacterial, antioxidant, and cytocompatibility assessments were conducted. The 2% AgNPs composite exhibited pronounced antimicrobial activity against Gram\u2010positive (\n                    <jats:italic>L. monocytogenes, S. aureus<\/jats:italic>\n                    ), Gram\u2010negative (\n                    <jats:italic>E. coli, Salmonella<\/jats:italic>\n                    sp.), and fungal strain (\n                    <jats:italic>Candida albicans<\/jats:italic>\n                    ). The 2% AgNPs hydrogel was cytocompatible with normal Vero cells (IC\n                    <jats:sub>50<\/jats:sub>\n                    \u00a0=\u00a0599.9\u00a0\u00a0\u00b1\u00a0\u00a03.47\u00a0\u00b5g\/mL), while the 8% AgNPs variant demonstrated potent antioxidant capacity (IC\n                    <jats:sub>50<\/jats:sub>\n                    of 21.77\u00a0\u00b5g\/mL and 33.3\u00a0\u00b5g\/mL via DPPH and ABTS assays, respectively). Molecular dynamics simulations confirmed strong interactions between hydrogel components and key binding site residues, ensuring composite stability. These results support the potential of AgNPs\u2010enhanced SA\/KC hydrogels for antimicrobial wound dressings, bioactive drug delivery matrices, and antioxidant\u2010active food packaging materials.\n                  <\/jats:p>","DOI":"10.1002\/biot.70117","type":"journal-article","created":{"date-parts":[[2025,9,15]],"date-time":"2025-09-15T13:40:47Z","timestamp":1757943647000},"source":"Crossref","is-referenced-by-count":6,"title":["Exploring the Activities of Alginate\u2010Carrageenan@AgNPs Hydrogels Supported by Molecular Docking Studies for Potential Antimicrobial and Antioxidant Agents"],"prefix":"10.1002","volume":"20","author":[{"given":"Ghada E.","family":"Dawwam","sequence":"first","affiliation":[{"name":"Botany and Microbiology Department Faculty of Science Benha University  Benha Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4252-8175","authenticated-orcid":false,"given":"Ahmed M.","family":"Khalil","sequence":"additional","affiliation":[{"name":"Photochemistry Department National Research Centre  Dokki Giza Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed A.","family":"El\u2010Rashedy","sequence":"additional","affiliation":[{"name":"Chemistry of Natural and Microbial Products Department National Research Centre  Dokki Giza Egypt"},{"name":"Department of Organic and Medicinal Chemistry Faculty of Pharmacy University of Sadat City  Menoufia Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Doaa M.","family":"Azmy","sequence":"additional","affiliation":[{"name":"(Project Member) at Cellulose and Paper Department National Research Centre  Dokki Giza Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7971-4318","authenticated-orcid":false,"given":"Samir","family":"Kamel","sequence":"additional","affiliation":[{"name":"(Project Member) at Cellulose and Paper Department National Research Centre  Dokki Giza Egypt"},{"name":"Cellulose and Paper Department National Research Centre  Dokki Giza Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,9,15]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/polym13030354"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1039\/D4RA06818A"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.202211323"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/biot.202300093"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodhyd.2025.111343"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11694-025-03156-2"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2023.125872"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/825203"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/stresses3030039"},{"key":"e_1_2_9_11_1","article-title":"Recent Advances in Alginate\u2010Based Hydrogels for the Adsorption\u2013Desorption of Heavy Metal Ions From Water: A Review","author":"Chen M.","year":"2024","journal-title":"Separation and Purification Technology"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/polym15112511"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/colloids6040078"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2023.125357"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fbio.2022.101579"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2024.e35264"},{"key":"e_1_2_9_17_1","unstructured":"C. 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