{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T00:51:05Z","timestamp":1779238265620,"version":"3.51.4"},"reference-count":16,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,11,20]],"date-time":"2025-11-20T00:00:00Z","timestamp":1763596800000},"content-version":"vor","delay-in-days":19,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotechnology Journal"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Increasing evidence supports the human sodium\u2010coupled citrate transporter (hNaCT) as a potential therapeutic target for metabolic syndrome and early infantile epileptic encephalopathy type 25 (EIEE25). While isotopic tracers remain the reference method for evaluating citrate transport, the need for specialized equipment and regulatory approval restricts their widespread use. Here, we report the development and validation of a robust, fluorescent\u2010based assay to evaluate hNaCT\u2010mediated citrate transport in live cells at both single\u2010cell and high\u2010throughput levels. This method utilizes a baculoviral vector to modify HEK293 cells to co\u2010express a genetically encoded citrate sensor (Citron1) and the hNaCT. This cell\u2010based platform enabled real\u2010time monitoring of citrate transport using fluorescent microscopy and a standard multiwell plate reader. A key strength of this approach is its ability to assess citrate transport in the same cells before and after experimental interventions. Accordingly, this approach enables the functional characterization of hNaCT, including its pharmacological inhibitors and genetic variants with altered activity. Overall, the method provides a reliable assessment of citrate transport and offers a versatile platform suitable for identifying novel lead compounds for the therapeutic modulation of hNaCT.<\/jats:p>","DOI":"10.1002\/biot.70156","type":"journal-article","created":{"date-parts":[[2025,11,20]],"date-time":"2025-11-20T09:09:53Z","timestamp":1763629793000},"source":"Crossref","is-referenced-by-count":1,"title":["A Fluorescent\u2010Based Method to Assess the Activity of the Human Sodium\u2010Coupled Citrate Transporter"],"prefix":"10.1002","volume":"20","author":[{"given":"Natali","family":"C\u00e1rcamo\u2010Lemus","sequence":"first","affiliation":[{"name":"Centro De Estudios Cient\u00edficos (CECs)  Valdivia Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angelo","family":"Bernier","sequence":"additional","affiliation":[{"name":"Centro De Estudios Cient\u00edficos (CECs)  Valdivia Chile"},{"name":"Universidad Austral de Chile (UACh)  Valdivia Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ivana","family":"Oporto\u2010Ortega","sequence":"additional","affiliation":[{"name":"Centro De Estudios Cient\u00edficos (CECs)  Valdivia Chile"},{"name":"Universidad Austral de Chile (UACh)  Valdivia Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alejandro San","family":"Mart\u00edn","sequence":"additional","affiliation":[{"name":"Centro De Estudios Cient\u00edficos (CECs)  Valdivia Chile"},{"name":"Facultad De Medicina Universidad San Sebasti\u00e1n  Valdivia Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6514-3980","authenticated-orcid":false,"given":"Pamela Y.","family":"Sandoval","sequence":"additional","affiliation":[{"name":"Centro De Estudios Cient\u00edficos (CECs)  Valdivia Chile"},{"name":"Facultad De Medicina Universidad San Sebasti\u00e1n  Valdivia Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,11,20]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu11112576"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M207072200"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.290.5499.2137"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/metabo12080732"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2011.06.009"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.2119\/molmed.2016.00077"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jmedchem.3c00179"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2024.168820"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/acscentsci.0c00518"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1177\/108705719900400206"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010018\u201029547\u20108"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jmedchem.5b01752"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1042\/BCJ20200592"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj20030827"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.adx3011"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22010148"}],"container-title":["Biotechnology Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/biot.70156","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/biot.70156","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/biot.70156","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T12:21:21Z","timestamp":1772972481000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/biot.70156"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11]]},"references-count":16,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2025,11]]}},"alternative-id":["10.1002\/biot.70156"],"URL":"https:\/\/doi.org\/10.1002\/biot.70156","archive":["Portico"],"relation":{},"ISSN":["1860-6768","1860-7314"],"issn-type":[{"value":"1860-6768","type":"print"},{"value":"1860-7314","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11]]},"article-number":"e70156"}}