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This study used a combined decellularization method with sodium dodecyl sulfate (SDS), Triton X\u2010100, and DNase to process porcine myocardial tissue (PMT), yielding decellularized extracellular matrix (dECM). Quantitative analysis revealed that the dECM contained collagen, DNA, and glycosaminoglycans (GAGs) at concentrations of 2.63\u00a0\u00b1\u00a00.37 \u00b5g\/mg, 4.27\u00a0\u00b1\u00a00.79 ng\/mg, and 15.94\u00a0\u00b1\u00a00.60 \u00b5g\/mg. Additionally, a conductive hydrogel patch (PDA\/CSCA\/PAM) with a uniform porous structure and excellent mechanical properties was developed. Its adhesive strengths on glass, stainless steel, and PMT were 50.71\u00a0\u00b1\u00a02.88 kPa, 34.19\u00a0\u00b1\u00a03.63 kPa, and 54.71\u00a0\u00b1\u00a03.24 kPa, respectively. Bacterial inhibition rates reached 103.26\u00a0\u00b1\u00a03.52% (Day 1) and 99.26\u00a0\u00b1\u00a05.35% (Day 3), indicating significant antimicrobial efficacy. The patch met myocardial tissue engineering standards for swelling ratio, hydrophilicity (contact angle &lt;90\u00b0), hemolysis rate (&lt;5%), and conductivity (10\n                    <jats:sup>\u22124<\/jats:sup>\n                    S cm\n                    <jats:sup>\u22121)<\/jats:sup>\n                    , with biosafety certified by ISO 10993\u20105. These results highlight its mechanical compatibility, antimicrobial activity, and biocompatibility, offering a multifunctional solution for myocardial infarction repair with high clinical translation potential.\n                  <\/jats:p>","DOI":"10.1002\/biot.70169","type":"journal-article","created":{"date-parts":[[2025,12,13]],"date-time":"2025-12-13T14:05:41Z","timestamp":1765634741000},"source":"Crossref","is-referenced-by-count":0,"title":["A 3D Bioprinted PDA\/CSCA\/PAM Hybrid Hydrogel Patch for Myocardial Infarction Healing"],"prefix":"10.1002","volume":"20","author":[{"given":"Yiran","family":"Tang","sequence":"first","affiliation":[{"name":"Cancer Hospital of Dalian University of Technology Dalian R&amp;D Center for Stem Cell and Tissue Engineering Dalian University of Technology  Dalian China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie","family":"Xu","sequence":"additional","affiliation":[{"name":"Cancer Hospital of Dalian University of Technology 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