{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T04:21:17Z","timestamp":1773030077180,"version":"3.50.1"},"reference-count":39,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,2,5]],"date-time":"2026-02-05T00:00:00Z","timestamp":1770249600000},"content-version":"vor","delay-in-days":4,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2022YFD1201600"],"award-info":[{"award-number":["2022YFD1201600"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotechnology Journal"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Hyoscyamine, an active tropane alkaloid primarily extracted from the medicinal plant\n                    <jats:italic>Atropa belladonna<\/jats:italic>\n                    , exhibits analgesic and sedative effects and is widely used as a precursor in pharmaceutical formulations. However, the low yield of hyoscyamine and the complex composition of tropane alkaloids in wild\u2010type\n                    <jats:italic>A. belladonna<\/jats:italic>\n                    plants pose significant challenges for industrial production, making it imperative to modify the metabolic pathway to enhance hyoscyamine yield and purity. In this study, a pull\u2010block metabolic engineering strategy, which enhanced hyoscyamine biosynthesis by co\u2010overexpressing\n                    <jats:italic>AbPYKS<\/jats:italic>\n                    ,\n                    <jats:italic>AbCYP82M3<\/jats:italic>\n                    ,\n                    <jats:italic>AbUGT1<\/jats:italic>\n                    ,\n                    <jats:italic>AbLS<\/jats:italic>\n                    , and\n                    <jats:italic>AbHDH<\/jats:italic>\n                    genes and blocked the metabolic diversion of hyoscyamine by deleting the\n                    <jats:italic>AbH6H<\/jats:italic>\n                    gene, was employed. During this process, the glyphosate resistance gene\n                    <jats:italic>G2\u2010EPSPS<\/jats:italic>\n                    was introduced into\n                    <jats:italic>A. belladonna<\/jats:italic>\n                    . Ultimately, the engineered plants with glyphosate resistance were obtained, which showed significantly enhanced hyoscyamine yield (6.511\u00a0vs. 0.989\u00a0mg\/g DW in leaves) and purity (the absence of anisodamine and scopolamine) relative to the wild\u2010type. These results not only enrich the germplasm resources of\n                    <jats:italic>A. belladonna<\/jats:italic>\n                    but also hold promise for advancing the production of the hyoscyamine\u2010based pharmaceuticals. Next, we will evaluate these engineered plants under field conditions for both hyoscyamine production and growth\/development.\n                  <\/jats:p>","DOI":"10.1002\/biot.70187","type":"journal-article","created":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T07:19:58Z","timestamp":1770362398000},"source":"Crossref","is-referenced-by-count":0,"title":["A Pull\u2010Block Metabolic Engineering Strategy for High\u2010Yield and High\u2010Purity Hyoscyamine Production in\n                    <i>Atropa belladonna<\/i>"],"prefix":"10.1002","volume":"21","author":[{"given":"Lingjiang","family":"Zeng","sequence":"first","affiliation":[{"name":"School of Life Sciences Southwest University  Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongting","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Life Sciences Southwest University  Chongqing 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Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0140-4192","authenticated-orcid":false,"given":"Min","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Pharmaceutical Sciences Southwest University  Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhihua","family":"Liao","sequence":"additional","affiliation":[{"name":"School of Life Sciences Southwest University  Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-6282-1700","authenticated-orcid":false,"given":"Sheng","family":"Tong","sequence":"additional","affiliation":[{"name":"School of Life Sciences Southwest University  Chongqing 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