{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T16:36:46Z","timestamp":1793205406227,"version":"4.5.0"},"reference-count":31,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T00:00:00Z","timestamp":1773878400000},"content-version":"vor","delay-in-days":18,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotechnology Journal"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The global SARS\u2010CoV\u20102 pandemic has underlined the urgent need for effective vaccine platforms. Adenoviral vectors have gained attention due to their high transgene capacity, broad tissue tropism, and innate immunostimulatory properties. This study aimed to develop and evaluate a recombinant adenoviral vaccine, Ad5Spike, encoding the full\u2010length SARS\u2010CoV\u20102 Spike glycoprotein. The Ad5Spike vector was generated using Gateway Cloning Technology and produced by transient calcium phosphate\u2010mediated transfection of 293A cells. Viral particles (VP) were purified via CsCl density gradient ultracentrifugation. Female BALB\/c mice (6\u20138 weeks old,\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u00a05 per group per timepoint) were immunized intraperitoneally with 10\n                    <jats:sup>8<\/jats:sup>\n                    , 10\n                    <jats:sup>10<\/jats:sup>\n                    , or 10\n                    <jats:sup>1<\/jats:sup>\n                    <jats:sup>2<\/jats:sup>\n                    viral particles. Humoral and cellular immune responses were evaluated at 30\u2010 and 90\u2010days post\u2010immunization using ELISA, ELISpot, and pseudovirus neutralization assays. Ad5Spike vaccination induced measurable anti\u2010Spike IgG responses, with persistent antibody levels observed up to 90 days. Splenocyte analysis revealed elevated IFN\u2010\u03b3, TNF\u2010\u03b1, and IL\u20102 secretion, consistent with initial humoral and cellular activation. Neutralizing antibody activity against a lentiviral pseudovirus bearing the SARS\u2010CoV\u20102 Spike (Wuhan\u20101) was dose\u2010dependent and highest in the 10\n                    <jats:sup>1<\/jats:sup>\n                    <jats:sup>2<\/jats:sup>\n                    group. In conclusion, this early preclinical study demonstrates that the Ad5Spike vaccine elicited detectable humoral and cellular immune responses, providing a proof\u2010of\u2010concept for the immunogenicity of this adenoviral\u2010based platform.\n                  <\/jats:p>","DOI":"10.1002\/biot.70216","type":"journal-article","created":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T08:45:27Z","timestamp":1773909927000},"source":"Crossref","is-referenced-by-count":2,"title":["An Ad5\u2010Based COVID\u201019 Vaccine Encoding SARS\u2010CoV\u20102 Spike Glycoprotein Induces Measurable Antibody and Cytokine Responses in Mice"],"prefix":"10.1002","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0726-803X","authenticated-orcid":false,"given":"Fulya","family":"Erendor","sequence":"first","affiliation":[{"name":"Department of Gene and Cell Therapy Faculty of Medicine Akdeniz University Antalya T\u00fcrkiye"},{"name":"Department of Medical Biology Faculty of Medicine Ordu University Ordu T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatih","family":"Uzer","sequence":"additional","affiliation":[{"name":"Department of Gene and Cell Therapy Faculty of Medicine Akdeniz University Antalya T\u00fcrkiye"},{"name":"Department of Respiratory Diseases Akdeniz University Medical School Antalya T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8379-5678","authenticated-orcid":false,"given":"Salih","family":"Sanlioglu","sequence":"additional","affiliation":[{"name":"Department of Gene and Cell Therapy Faculty of Medicine Akdeniz University Antalya 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