{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T13:17:51Z","timestamp":1777641471051,"version":"3.51.4"},"reference-count":30,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T00:00:00Z","timestamp":1776384000000},"content-version":"vor","delay-in-days":16,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotechnology Journal"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Virus removal filtration constitutes an essential component in biopharmaceutical manufacturing processes for both recombinant and plasma\u2010derived protein products. The increasing demand for protein\u2010based therapies necessitates expedited analytical screening methodologies for virus filtration materials and process parameters utilizing high\u2010throughput development (HTPD) platforms. This study examines a novel HTPD methodology, specifically the implementation of analytical centrifugal ultrafiltration, as a scaled\u2010down virus removal filtration tool. A nanocellulose\u2010derived filter membrane of controlled porosity (33\u00a0\u00b5m thickness, 23\u00a0nm) was integrated into a centrifugal ultrafiltration apparatus. The system underwent comprehensive evaluation to determine centrifugal ultrafiltration flux characteristics, product yield across varying buffer viscosities, virus removal capacity utilizing model MS2 (27\u00a0nm) and \u03a6X174 (28\u00a0nm) bacteriophages, and IgG throughput capacity. Application of centrifugal force facilitated the generation of multiple filtration pressure gradients suitable for small\u2010volume sample processing. The resultant flux decay profiles exhibited high reproducibility, demonstrating superior IgG throughput and virus removal efficiency. Overall, the approach adds value to the arsenal of analytical tools useful for HTPD in biopharmaceutics.<\/jats:p>","DOI":"10.1002\/biot.70227","type":"journal-article","created":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T10:50:15Z","timestamp":1776423015000},"source":"Crossref","is-referenced-by-count":0,"title":["Analytical Centrifugal Ultrafiltration as a Tool for High Throughput Process Development in Virus Removal Filtration"],"prefix":"10.1002","volume":"21","author":[{"given":"Sebastien","family":"Maffeis","sequence":"first","affiliation":[{"name":"Nanotechnology and Functional Materials Department of Materials Science and Engineering Uppsala University  Uppsala Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuliia","family":"Fomichova","sequence":"additional","affiliation":[{"name":"Pharmaceutical Technology Department of Pharmaceutical Biosciences Uppsala University  Uppsala Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Levon","family":"Manukyan","sequence":"additional","affiliation":[{"name":"Nanotechnology and Functional Materials Department of Materials Science and Engineering Uppsala University  Uppsala Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8105-2317","authenticated-orcid":false,"given":"Albert","family":"Mihranyan","sequence":"additional","affiliation":[{"name":"Nanotechnology and Functional Materials Department of Materials Science and Engineering Uppsala University  Uppsala Sweden"},{"name":"Pharmaceutical Technology Department of Pharmaceutical Biosciences Uppsala University  Uppsala Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,4,17]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.memsci.2020.118330"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/btpr.415"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/biot.70161"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.memsci.2025.124266"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scib.2018.04.014"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00589301"},{"key":"e_1_2_10_8_1","first-page":"353","article-title":"Konzentrirung von Serum durch Ultrafiltration Mittels der Zentrifuge","volume":"197","author":"Toth A.","year":"1928","journal-title":"Biochemische Zeitschrift"},{"key":"e_1_2_10_9_1","first-page":"355","article-title":"Ultrafiltration kleiner Fl\u00fcssigkeitsmengen mittels der Zentrifuge","volume":"191","author":"Toth A.","year":"1927","journal-title":"Biochemische Zeitschrift"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01752896"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/12.6_ts.68"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.3.1.67"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02160557"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1039\/TF9373301094"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.4.2.240"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.memsci.2011.02.022"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.memsci.2017.03.043"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1039\/C6MH00090H"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsanm.9b01351"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biologicals.2015.08.001"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.6b03093"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.3390\/biomedicines8040069"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.3389\/frmst.2024.1338366"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1021\/acssuschemeng.9b03905"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/gch2.201870174"},{"key":"e_1_2_10_26_1","first-page":"52","article-title":"Advanced Viral Clearance Study Design: A Total Viral Challenge Approach to Virus Filtration","volume":"16","author":"Burnham M.","year":"2018","journal-title":"Bioprocess International"},{"key":"e_1_2_10_27_1","doi-asserted-by":"crossref","volume-title":"Fluid Sterilization by Filtration","author":"Johnston P. 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