{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T14:15:56Z","timestamp":1784556956897,"version":"3.55.0"},"reference-count":39,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T00:00:00Z","timestamp":1778112000000},"content-version":"vor","delay-in-days":6,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2022YFC2106100"],"award-info":[{"award-number":["2022YFC2106100"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotechnology Journal"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    \u03b2\u2010Ionone, prized for its characteristic violet aroma and woody undertones, has emerged as a valuable ingredient in the fragrance industry. Beyond its aromatic qualities, growing interest in its diverse biological activities has positioned it as a compound of relevance in the biopharmaceutical field. As production strategies shift from traditional chemical synthesis toward more sustainable biological routes, this study explores the engineered biosynthesis of \u03b2\u2010ionone in\n                    <jats:italic>Yarrowia lipolytica<\/jats:italic>\n                    . Through systematic combinatorial metabolic engineering, a strain capable of accumulating 743\u00a0mg\/L of \u03b2\u2010carotene was constructed. Heterologous expression of the petunia\u2010derived carotenoid cleavage dioxygenase gene PhCCD1 enabled efficient conversion of \u03b2\u2010carotene to \u03b2\u2010ionone, yielding 137\u00a0mg\/L in shake\u2010flask cultures. Subsequent integration of the isoprene alcohol metabolic pathway further enhanced precursor supply, boosting \u03b2\u2010ionone production to 236.7\u00a0mg\/L in shake flasks. In a 5 L bioreactor, optimization of the culture medium and feeding strategy led to a final titer of 624.7\u00a0mg\/L. Collectively, these results establish a promising and replicable strategy for developing microbial cell factories capable of efficient \u03b2\u2010ionone synthesis.\n                  <\/jats:p>","DOI":"10.1002\/biot.70230","type":"journal-article","created":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T10:57:45Z","timestamp":1778151465000},"source":"Crossref","is-referenced-by-count":0,"title":["Engineering Modification for the Efficient Synthesis of \u03b2\u2010ionone by\n                    <i>Yarrowia lipolytica<\/i>"],"prefix":"10.1002","volume":"21","author":[{"given":"Kai","family":"Gu","sequence":"first","affiliation":[{"name":"Science Center for Future Foods Jiangnan University  Wuxi China"},{"name":"Key Laboratory of Industrial Biotechnology School of Biotechnology Ministry of Education Jiangnan University  Wuxi China"},{"name":"Jiangsu Province Basic Research Center For Synthetic Biology Jiangnan University  Wuxi 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