{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T13:04:14Z","timestamp":1784552654576,"version":"3.55.0"},"reference-count":35,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T00:00:00Z","timestamp":1780358400000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotechnology Journal"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:bold>Background<\/jats:bold>\n                    : Chronic constipation is prevalent in middle\u2010aged and elderly individuals and can markedly impair quality of life and increase comorbidity risk. However, safe and effective therapies for this population remain limited because of age\u2010related physiological changes, polypharmacy, and concerns about long\u2010term medication use.\n                  <\/jats:p>\n                  <jats:p>\n                    Methods: We conducted a randomized, double\u2010blind, controlled clinical trial enrolling 140 participants aged \u226545 years with chronic constipation to evaluate a synbiotic containing\n                    <jats:italic>Heyndrickxia coagulans<\/jats:italic>\n                    (\n                    <jats:italic>H. coagulans<\/jats:italic>\n                    ) TBC169 plus dietary fibers as an adjunct intervention.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Results<\/jats:bold>\n                    : Compared with dietary fiber alone, the TBC169\u2010based synbiotic significantly improved constipation outcomes, including increased weekly bowel movements (WBMs), higher Bristol stool scale (BSS) scores, and reduced Wexner and Patient Assessment of Constipation Quality of Life (PAC\u2010QOL) scores. Post\u2010intervention gut microbiota profiling showed higher Shannon and Faith_PD diversity indices and distinct community composition in the synbiotic group compared with the dietary\u2010fiber control group, along with higher relative abundance of\n                    <jats:italic>Blautia<\/jats:italic>\n                    ,\n                    <jats:italic>Akkermansia<\/jats:italic>\n                    ,\n                    <jats:italic>Faecalibacterium<\/jats:italic>\n                    , and\n                    <jats:italic>Bacillus<\/jats:italic>\n                    and lower relative abundance of\n                    <jats:italic>Collinsella<\/jats:italic>\n                    . Because baseline faecal samples were not collected, these microbiota findings should be interpreted as exploratory between\u2010group associations. Ecological analyses suggested enhanced microbial stability and metabolic potential, and correlations linked key taxa with clinical improvement. The intervention was well\u2010tolerated, with only mild, transient adverse events.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Conclusions<\/jats:bold>\n                    : The synbiotic formulation containing\n                    <jats:italic>H. coagulans<\/jats:italic>\n                    TBC169 and dietary fiber\u2010related components was well\u2010tolerated and may improve constipation\u2010related outcomes in middle\u2010aged and elderly adults. Because the formulation contained both probiotic and fiber components, the observed effects should be attributed to the combined synbiotic product rather than to\n                    <jats:italic>H. coagulans<\/jats:italic>\n                    TBC169 alone.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Trial Registration<\/jats:bold>\n                    : Chinese Clinical Trial Registry (ChiCTR2400085844,\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.chictr.org.cn\/\">http:\/\/www.chictr.org.cn\/<\/jats:ext-link>\n                    ), registered on June 19, 2024.\n                  <\/jats:p>","DOI":"10.1002\/biot.70250","type":"journal-article","created":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T07:10:53Z","timestamp":1780384253000},"source":"Crossref","is-referenced-by-count":0,"title":["Synbiotic Supplementation With\n                    <i>Heyndrickxia coagulans<\/i>\n                    TBC169 Improves Chronic Constipation in Middle\u2010Aged and Elderly"],"prefix":"10.1002","volume":"21","author":[{"given":"Xiaoni","family":"Zhou","sequence":"first","affiliation":[{"name":"Department of Obstetrics &amp; Gynecology the Second People's Hospital of Jingdezhen  Jingdezhen Jiangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Canhui","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Geriatrics the Second Affiliated Hospital of Nanchang University Jiangxi Medical College Nanchang University  Nanchang Jiangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gang","family":"Hu","sequence":"additional","affiliation":[{"name":"School of Life Sciences Nanchang University  Nanchang Jiangxi China"},{"name":"National Engineering Research Center for Bioengineering Drugs and the Technologies Jiangxi Province Key Laboratory of Bioengineering Drugs Institute of Translational 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