{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T08:00:01Z","timestamp":1785398401665,"version":"3.56.0"},"reference-count":36,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T00:00:00Z","timestamp":1783555200000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001407","name":"Department of Biotechnology, Ministry of Science and Technology, India","doi-asserted-by":"publisher","award":["BT\/PR25180\/AAQ\/1\/695\/2017"],"award-info":[{"award-number":["BT\/PR25180\/AAQ\/1\/695\/2017"]}],"id":[{"id":"10.13039\/501100001407","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotechnology Journal"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Assisted reproductive technologies have become crucial in addressing the growing global demand for livestock production. Follicle\u2010stimulating hormone (FSH) is essential for superovulation in ART procedures. Animal pituitary\u2010derived FSH is widely utilized for these purposes; however, it is costly and presents other limitations. Currently, there are very few commercially available recombinant bovine FSH products, primarily due to challenges in equimolar expression of the FSH\u03b1 and FSH\u03b2 subunits, inefficient post\u2010translational modifications that result in improper heterodimerization, and loss of bioactivity. This study, for the first time, reports the isolation and cloning of FSH\u03b1 and FSH\u03b2 cDNA from Indian cattle (\n                    <jats:italic>Bos indicus<\/jats:italic>\n                    ) into a bicistronic\u2010mammalian vector, co\u2010expressed using the P2A peptide. This approach facilitates simultaneous expression and proper dimerization in an in vitro system, producing bioactive recombinant FSH. Additionally, we identified a single\u2010nucleotide\u2010polymorphism (SNP) in the FSH\u03b2 region, resulting in a Serine\u2010to\u2010Glycine substitution, unique to\n                    <jats:italic>Bos indicus<\/jats:italic>\n                    . Notably, this SNP was found to be conserved across Indian cattle breeds. This study successfully demonstrates, for the first time, the efficient in vitro production of FSH, thereby overcoming the challenges associated with large\u2010scale commercial production. This advancement will aid in the production of indigenous recombinant bovine FSH, enhancing outcomes in cattle breeding.\n                  <\/jats:p>","DOI":"10.1002\/biot.70279","type":"journal-article","created":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T11:08:02Z","timestamp":1783595282000},"source":"Crossref","is-referenced-by-count":0,"title":["Engineering a Bicistronic Expression System for Mammalian Cell\u2010Based Production of Biofunctional Recombinant Follicle Stimulating Hormone (FSH) of\n                    <i>Bos Indicus<\/i>\n                    Origin"],"prefix":"10.1002","volume":"21","author":[{"given":"Abhishek","family":"Das","sequence":"first","affiliation":[{"name":"Gene and Protein Engineering Lab National Institute of Animal Biotechnology  Hyderabad Telangana India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Srimoyee","family":"Koner","sequence":"additional","affiliation":[{"name":"Gene and Protein Engineering Lab National Institute of Animal Biotechnology  Hyderabad Telangana India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Venkateswaran","family":"Ganesan","sequence":"additional","affiliation":[{"name":"Gene and Protein Engineering Lab National Institute of Animal Biotechnology  Hyderabad Telangana India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dewanshu","family":"Sharma","sequence":"additional","affiliation":[{"name":"Gene and Protein Engineering Lab National Institute of Animal Biotechnology  Hyderabad Telangana India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meenakshi","family":"Mansukhani","sequence":"additional","affiliation":[{"name":"Gene and Protein Engineering Lab National Institute of Animal Biotechnology  Hyderabad Telangana India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amit","family":"Pal","sequence":"additional","affiliation":[{"name":"Gene and Protein Engineering Lab National Institute of Animal 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Sharma","sequence":"additional","affiliation":[{"name":"Reproductive Endocrinology Lab National Institute of Animal Biotechnology  Hyderabad Telangana India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Subeer S.","family":"Majumdar","sequence":"additional","affiliation":[{"name":"Gene and Protein Engineering Lab National Institute of Animal Biotechnology  Hyderabad Telangana India"},{"name":"Gujarat Biotechnology University  Gandhinagar Gujarat India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nirmalya","family":"Ganguli","sequence":"additional","affiliation":[{"name":"Gene and Protein Engineering Lab National Institute of Animal Biotechnology  Hyderabad Telangana India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,9]]},"reference":[{"key":"e_1_2_12_2_1","volume-title":"StatPearls","author":"Orlowski 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