{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T08:01:03Z","timestamp":1785398463303,"version":"3.56.0"},"reference-count":45,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T00:00:00Z","timestamp":1784764800000},"content-version":"vor","delay-in-days":22,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotechnology Journal"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The rapid expansion of the global seafood processing industry has generated substantial quantities of shrimp shells and other chitin\u2010rich waste. Recombinant chitinases provide a promising strategy for converting this biomass into high\u2010value chitin oligosaccharides. In this study, we systematically optimized the secretory expression of the GH18 exochitinase CtChi70 in\n                    <jats:italic>Komagataella phaffii<\/jats:italic>\n                    (formerly\n                    <jats:italic>Pichia pastoris<\/jats:italic>\n                    ) and examined its applicability for the enzymatic conversion of shrimp shell\u2010derived chitin into N,N'\u2010diacetyl\u2010chitobiose ((GlcNAc)\n                    <jats:sub>2<\/jats:sub>\n                    ). Through signal peptide optimization, gene copy number amplification, and\n                    <jats:italic>HAC1<\/jats:italic>\n                    overexpression, extracellular chitinase activity in shake\u2010flask cultures reached 181\u00a0U\/L. Scale\u2010up using high\u2010cell\u2010density fed\u2010batch fermentation in a 7\u2010L bioreactor further increased activity to 2151\u00a0U\/L. A mild chitin extraction process was established using citric acid demineralization and low\u2010concentration NaOH deproteinization, yielding a suitable substrate for enzymatic hydrolysis. The resulting chitin was successfully converted into high\u2010purity (GlcNAc)\n                    <jats:sub>2<\/jats:sub>\n                    within 24\u00a0h. Collectively, this work establishes an efficient expression strategy for CtChi70 production and demonstrates an integrated, environmentally sustainable approach for shrimp shell valorization.\n                  <\/jats:p>","DOI":"10.1002\/biot.70284","type":"journal-article","created":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T14:55:38Z","timestamp":1784818538000},"source":"Crossref","is-referenced-by-count":0,"title":["Recombinant Expression of Chitinase CtChi70 in\n                    <i>Komagataella phaffii<\/i>\n                    for Sustainable N, N'\u2010Diacetyl\u2010Chitobiose Production From Shrimp Shells"],"prefix":"10.1002","volume":"21","author":[{"given":"Qinghe","family":"Chu","sequence":"first","affiliation":[{"name":"Guangdong Engineering Research Center of Low\u2010Carbon Synthetic Biotechnology School of Biology and Biological Engineering South China University of Technology  Guangzhou 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