{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T08:00:46Z","timestamp":1785398446261,"version":"3.56.0"},"reference-count":72,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T00:00:00Z","timestamp":1785283200000},"content-version":"vor","delay-in-days":28,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotechnology Journal"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>High\u2010grade serous ovarian cancer (HGSOC) exhibits pronounced intratumoral heterogeneity and chemoresistance, demanding experimental platforms that more accurately recapitulate stemness\u2010driven relapse. Despite increasing use of three\u2010dimensional (3D) systems in cancer research, their comparative translational performance in ovarian cancer remains unclear. Here, we systematically benchmark two scalable 3D platforms, scaffold\u2010free ultra\u2010low attachment (ULA) spheroids and extracellular matrix mimetic photopatterned gelatin methacryloyl (GelMA) hydrogels, using HGSOC cell lines and patient\u2010derived primary samples. Under matched conditions, both systems enhanced cancer stem cell (CSC) associated phenotypes relative to 2D culture, including induction of stemness markers, CSC surface populations, epithelial to mesenchymal transition signatures, and drug resistance. ULA enabled long\u2010term spheroid maturation and robust expansion of intrinsically stem\u2010like populations, whereas GelMA photopatterning provided spatially defined microenvironmental control that promoted rapid and uniform spheroid formation and efficiently induced stem\u2010like traits in low\u2010stemness models. Notably, baseline SOX2 expression partially correlated with chemoresistance, supporting the biological relevance of CSC enriched platforms. Collectively, this platform level comparison establishes complementary, biotechnology driven strategies for reproducible modeling of HGSOC stemness and therapy resistance, with implications for translational drug evaluation and precision oncology.<\/jats:p>","DOI":"10.1002\/biot.70287","type":"journal-article","created":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T06:06:03Z","timestamp":1785391563000},"source":"Crossref","is-referenced-by-count":0,"title":["Benchmarking Ultra\u2010Low Attachment and Photopatterned GelMA 3D Culture Platforms for Modeling Cancer Stemness in High\u2010Grade Serous Ovarian Cancer"],"prefix":"10.1002","volume":"21","author":[{"given":"Deren Demirel","family":"Yavuz","sequence":"first","affiliation":[{"name":"Koc University Graduate School of Health Sciences  Istanbul Turkey"},{"name":"Koc University Research Center for Translational Medicine (KUTTAM)  Istanbul 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Merve","family":"Aydin","sequence":"additional","affiliation":[{"name":"Koc University Graduate School of Health Sciences  Istanbul Turkey"},{"name":"Koc University Research Center for Translational Medicine (KUTTAM)  Istanbul Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Merve","family":"Kayis","sequence":"additional","affiliation":[{"name":"Koc University Graduate School of Health Sciences  Istanbul Turkey"},{"name":"Koc University Research Center for Translational Medicine (KUTTAM)  Istanbul Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Burak","family":"Giray","sequence":"additional","affiliation":[{"name":"Koc University School of Medicine  Istanbul Turkey"},{"name":"Koc University Hospital  Istanbul Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7831-7070","authenticated-orcid":false,"given":"Dogan","family":"Vatansever","sequence":"additional","affiliation":[{"name":"Koc 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