{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T11:14:47Z","timestamp":1788174887788,"version":"build-2803163510"},"reference-count":48,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,8,16]],"date-time":"2026-08-16T00:00:00Z","timestamp":1786838400000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22278188"],"award-info":[{"award-number":["22278188"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotechnology Journal"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Capsanthin is a high\u2010value tetraterpenoid widely utilized in the food, cosmetic, and pharmaceutical industries. However, its traditional production via plant extraction and chemical synthesis faces severe sustainability and cost constraints. The oleaginous yeast\n                    <jats:italic>Yarrowia lipolytica<\/jats:italic>\n                    (\n                    <jats:italic>Y. lipolytica<\/jats:italic>\n                    ) offers a superior chassis due to its robust mevalonate pathway and abundant lipid droplets for pigment sequestration. In this study, we systematically engineered\n                    <jats:italic>Y. lipolytica<\/jats:italic>\n                    for the de novo biosynthesis of capsanthin. By heterologously co\u2010expressing of\n                    <jats:italic>Capsicum annuum<\/jats:italic>\n                    capsanthin\/capsorubin synthase (CaCCS) and\n                    <jats:italic>Arabidopsis thaliana<\/jats:italic>\n                    zeaxanthin epoxidase (AtZEP), we achieved the first reported biosynthesis of capsanthin in\n                    <jats:italic>Y. lipolytica<\/jats:italic>\n                    . Combinatorial screening revealed that full\u2010length sequences of\n                    <jats:italic>CaCCS<\/jats:italic>\n                    and\n                    <jats:italic>AtZEP<\/jats:italic>\n                    yielded optimal production of 6.92\u00a0mg\/L. To further enhance the productivity of capsanthin, we evaluated spatial compartmentalization strategies. The results demonstrated that targeting the biosynthetic enzymes to the peroxisome via Ser\u2013Lys\u2013Leu (SKL) tags successfully increased the titer to 8.8\u00a0mg\/L by leveraging its specialized lipophilic and redox microenvironment. Finally, a 5\u2010L fed\u2010batch fermentation was conducted, achieving a maximum capsanthin titer of 48.15\u00a0mg\/L. This work provides a sustainable and scalable alternative to plant extraction and chemical synthesis for capsanthin production.\n                  <\/jats:p>","DOI":"10.1002\/biot.70291","type":"journal-article","created":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T06:20:58Z","timestamp":1786947658000},"source":"Crossref","is-referenced-by-count":0,"title":["Spatial Engineering of\n                    <i>Yarrowia lipolytica<\/i>\n                    for the Biosynthesis of Capsanthin"],"prefix":"10.1002","volume":"21","author":[{"given":"Jialei","family":"Zhang","sequence":"first","affiliation":[{"name":"National Engineering Research Center of Cereal Fermentation and Food Biomanufacturing Jiangnan University  Wuxi Jiangsu China"},{"name":"Key Laboratory of Industrial Biotechnology Ministry of Education and School of Biotechnology Jiangnan University  Wuxi Jiangsu China"},{"name":"Jiangsu Province Engineering Research Center of Food Synthetic Biotechnology Jiangnan University  Wuxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling","family":"Sun","sequence":"additional","affiliation":[{"name":"National Engineering Research Center of Cereal Fermentation and Food Biomanufacturing Jiangnan University  Wuxi Jiangsu China"},{"name":"Key Laboratory of Industrial Biotechnology Ministry of Education and School of Biotechnology Jiangnan University  Wuxi Jiangsu China"},{"name":"Jiangsu Province Engineering Research Center of Food Synthetic Biotechnology Jiangnan University  Wuxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1783-1658","authenticated-orcid":false,"given":"Liang","family":"Zhang","sequence":"additional","affiliation":[{"name":"National Engineering Research Center of Cereal Fermentation and Food Biomanufacturing Jiangnan University  Wuxi Jiangsu China"},{"name":"Key Laboratory of Industrial Biotechnology Ministry of Education and School of Biotechnology Jiangnan University  Wuxi Jiangsu China"},{"name":"Jiangsu Province Engineering Research Center of Food Synthetic Biotechnology Jiangnan University  Wuxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chongyang","family":"Din","sequence":"additional","affiliation":[{"name":"National Engineering Research Center of Cereal Fermentation and Food Biomanufacturing Jiangnan University  Wuxi Jiangsu China"},{"name":"Key Laboratory of Industrial Biotechnology Ministry of Education and School of Biotechnology Jiangnan University  Wuxi Jiangsu China"},{"name":"Jiangsu Province Engineering Research Center of Food Synthetic Biotechnology Jiangnan University  Wuxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenghua","family":"Gu","sequence":"additional","affiliation":[{"name":"National Engineering Research Center of Cereal Fermentation and Food Biomanufacturing Jiangnan University  Wuxi Jiangsu China"},{"name":"Key Laboratory of Industrial Biotechnology Ministry of 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