{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T11:15:05Z","timestamp":1788174905648,"version":"build-2803163510"},"reference-count":26,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T00:00:00Z","timestamp":1787184000000},"content-version":"vor","delay-in-days":19,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","award":["Bio\u00f6k_2275052"],"award-info":[{"award-number":["Bio\u00f6k_2275052"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","award":["Bio\u00f6k_2076199"],"award-info":[{"award-number":["Bio\u00f6k_2076199"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotechnology Journal"],"published-print":{"date-parts":[[2026,8]]},"abstract":"ABSTRACT<\/jats:title>\n \n Polyhydroxyalkanoates (PHAs), especially their copolymeric forms, such as poly(3\u2010hydroxybutyrate\u2010co\u20103\u2010hydroxyvalerate\u2010co\u20104\u2010hydroxyvalerate) (PHBVV), have promising properties for replacing petrochemical\u2010derived plastics. However, the research focuses mostly on producing PHA from a specific substrate or waste\u2010stream, while the reproducibility of a precise polymer composition is seldom targeted. This study systematically focuses on the influence of pressure conditions on the production of terpolymeric PHBVV by\n Cupriavidus necator<\/jats:italic>\n NCIMB 11599 using levulinic acid (LA). Three different process setups were evaluated to assess the impact of hydrostatic pressure (\u0394\n p<\/jats:italic>\n Hydro<\/jats:sub>\n ) and applied pressure (\u0394\n p<\/jats:italic>\n Vessel<\/jats:sub>\n ) compared to the process at ambient pressure. Differences were detected regarding carbon utilization (Y\n X\/Gluc<\/jats:sub>\n & Y\n PHA\/LA<\/jats:sub>\n ) and polymer composition. The Y\n X\/Gluc<\/jats:sub>\n increased in the pilot scale compared to the technical scale from 0.31\u00a0\u00b1\u00a00.01\u00a0g\n X<\/jats:sub>\n \u00a0g\n Gluc<\/jats:sub>\n \u22121<\/jats:sup>\n to 0.41\u00a0\u00b1\u00a00.01\u00a0g\n X<\/jats:sub>\n \u00a0g\n Gluc<\/jats:sub>\n \u22121<\/jats:sup>\n . Conversely, as \u0394\n p<\/jats:italic>\n Vessel<\/jats:sub>\n increased, the Y\n PHA\/LA<\/jats:sub>\n decreased from 0.35\u00a0\u00b1\u00a00.02\u00a0g\n PHA<\/jats:sub>\n \u00a0g\n LA<\/jats:sub>\n \u22121<\/jats:sup>\n to 0.23\u00a0\u00b1\u00a00.02\u00a0g\n PHA<\/jats:sub>\n \u00a0g\n LA<\/jats:sub>\n \u22121<\/jats:sup>\n , while the corresponding 3HV contents increased from 48\u00a0\u00b1\u00a01%\n (w\/w)<\/jats:sub>\n to 69\u00a0\u00b1\u00a02%\n (w\/w)<\/jats:sub>\n . These changes were consistent with pressure\u2010dependent variations in CO\n 2<\/jats:sub>\n concentration and a potentially increased carbon fixation via the energy\u2010intensive CBB cycle. This indicates that the polymer composition and cell vitality can be adjusted via the CO\n 2<\/jats:sub>\n concentration, a factor that should be taken into account when further scaling up of PHA production processes.\n <\/jats:p>","DOI":"10.1002\/biot.70298","type":"journal-article","created":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T07:03:06Z","timestamp":1787209386000},"source":"Crossref","is-referenced-by-count":0,"title":["Pressure Dependent Changes in CO\n 2<\/sub>\n \u2010Concentrations Modulate the 3\u2010Hydroxyvalerate Fraction in Terpolymeric Polyhydroxyalkanoates"],"prefix":"10.1002","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2105-8143","authenticated-orcid":false,"given":"Birk","family":"Achenbach","sequence":"first","affiliation":[{"name":"Institute of Interfacial Process Engineering and Plasma Technology IGVP University of Stuttgart Stuttgart Germany"},{"name":"Fraunhofer Institute for Interfacial Engineering and Biotechnology IGB Stuttgart Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5994-0254","authenticated-orcid":false,"given":"Pravesh","family":"Tamang","sequence":"additional","affiliation":[{"name":"Fraunhofer Institute for Interfacial Engineering and Biotechnology IGB Stuttgart Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2437-3405","authenticated-orcid":false,"given":"G\u00fcnter E. M.","family":"Tovar","sequence":"additional","affiliation":[{"name":"Institute of Interfacial Process Engineering and Plasma Technology IGVP University of Stuttgart Stuttgart Germany"},{"name":"Fraunhofer Institute for Interfacial Engineering and Biotechnology IGB Stuttgart Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5344-6549","authenticated-orcid":false,"given":"Susanne","family":"Zibek","sequence":"additional","affiliation":[{"name":"Institute of Interfacial Process Engineering and Plasma Technology IGVP University of Stuttgart Stuttgart Germany"},{"name":"Fraunhofer Institute for Interfacial Engineering and Biotechnology IGB Stuttgart Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,20]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2017.12.006"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/bioengineering9050204"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.10670"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1166\/jbmb.2009.1018"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiotec.2011.07.027"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1385\/ABAB:78:1\u20103:389"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0032\u20103861(92)90139\u2010N"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/polym14194226"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/app.41715"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep11617"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.69019"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpowsour.2024.235797"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/fermentation8030125"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/1751\u20107915.12149"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102672.2009.04631.x"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2023.108264"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253\u2010024\u201013284\u20102"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13068\u2010019\u20101489\u20105"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymben.2024.10.011"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/biot.70252"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jes.2018.09.001"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2022.913362"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biortech.2024.131214"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12934\u2010025\u201002840\u20108"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253\u2010015\u20107162\u20104"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep42717"}],"container-title":["Biotechnology Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/biot.70298","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/biot.70298","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/biot.70298","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T10:23:27Z","timestamp":1788171807000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/biot.70298"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8]]},"references-count":26,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2026,8]]}},"alternative-id":["10.1002\/biot.70298"],"URL":"https:\/\/doi.org\/10.1002\/biot.70298","archive":["Portico"],"relation":{},"ISSN":["1860-6768","1860-7314"],"issn-type":[{"value":"1860-6768","type":"print"},{"value":"1860-7314","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8]]},"article-number":"e70298"}}