{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T11:15:05Z","timestamp":1788174905635,"version":"build-2803163510"},"reference-count":44,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T00:00:00Z","timestamp":1787443200000},"content-version":"vor","delay-in-days":22,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100002347","name":"Bundesministerium f\u00fcr Bildung und Forschung","doi-asserted-by":"publisher","award":["13FH036KB2"],"award-info":[{"award-number":["13FH036KB2"]}],"id":[{"id":"10.13039\/501100002347","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotechnology Journal"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Stable expression of regulatory non\u2010coding RNAs enables targeted cell engineering in Chinese hamster ovary (CHO) cells but commonly relies on random integration, resulting in variable expression. Here, we establish a transposase\u2010mediated platform for stable microRNA (miRNA) expression in CHO cells and demonstrate the first application of a DNA transposon system for long\u2010term miRNA genome integration. Using miR\u20103096b\u20105p, we compared\n                    <jats:italic>p<\/jats:italic>\n                    <jats:italic>iggyBac<\/jats:italic>\n                    \u2010mediated integration with conventional random integration in antibody\u2010producing CHO cells. Transposase\u2010mediated integration enabled rapid generation of stable cell pools with higher fractions of GFP\u2010expressing cells. Both integration strategies supported comparable growth, viability, and antibody production in batch cultures, indicating that transposase\u2010mediated miRNA integration does not compromise bioprocess performance. At the molecular level, transposase\u2010mediated integration resulted in significantly higher transgene copy numbers and markedly increased miRNA expression, translating into stronger and more consistent regulation of the target genes\n                    <jats:italic>Fuk<\/jats:italic>\n                    and\n                    <jats:italic>Gmds<\/jats:italic>\n                    . By combining high integration efficiency and expression stability with the regulatory potential of miRNAs, this approach expands the applicability of transposase systems beyond protein\u2010coding transgenes and provides a robust platform for precise and durable post\u2010transcriptional gene regulation in CHO cell engineering.\n                  <\/jats:p>","DOI":"10.1002\/biot.70299","type":"journal-article","created":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T06:47:12Z","timestamp":1787554032000},"source":"Crossref","is-referenced-by-count":0,"title":["Expanding Transposase Technology to Regulatory Noncoding RNAs for Stable Expression in CHO Cells"],"prefix":"10.1002","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-0810-5913","authenticated-orcid":false,"given":"Sonja","family":"Lochmueller","sequence":"first","affiliation":[{"name":"Ulm University  Ulm Germany"},{"name":"Institute for Applied Biotechnology, University of Applied Sciences Biberach  Biberach Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6905-5283","authenticated-orcid":false,"given":"Linus","family":"Weiss","sequence":"additional","affiliation":[{"name":"Ulm University  Ulm Germany"},{"name":"Institute for Applied Biotechnology, University of Applied Sciences Biberach  Biberach Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kerstin","family":"Otte","sequence":"additional","affiliation":[{"name":"Institute for Applied Biotechnology, University of Applied Sciences Biberach  Biberach 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